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Literature summary extracted from

  • Goedl, C.; Griessler, R.; Schwarz, A.; Nidetzky, B.
    Structure-function relationships for Schizophyllum commune trehalose phosphorylase and their implications for the catalytic mechanism of family GT-4 glycosyltransferases (2006), Biochem. J., 397, 491-500.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.231 expression in Escherichia coli Schizophyllum commune

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.231 D379N ratio of phosphorolysis to hydrolysis decreases by a factor of 287500 Schizophyllum commune
2.4.1.231 H403A ratio of phosphorolysis to hydrolysis decreases by a factor of 2190 Schizophyllum commune
2.4.1.231 K512A ratio of phosphorolysis to hydrolysis decreases by a factor of 511111 Schizophyllum commune
2.4.1.231 R507A ratio of phosphorolysis to hydrolysis decreases by a factor of 575000 Schizophyllum commune

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.231 alpha-D-glucose 1-phosphate
-
 Schizophyllum commune
2.4.1.231 phosphate
-
 Schizophyllum commune
2.4.1.231 validoxylamine A
-
 Schizophyllum commune

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.231 0.8
-
 phosphate 30°C, pH 6.6, recombinant enzyme Schizophyllum commune
2.4.1.231 1.4
-
 phosphate 30°C, pH 6.6, natural enzyme Schizophyllum commune
2.4.1.231 1.6
-
 alpha-D-glucose 1-phosphate 30°C, pH 6.6, natural enzyme Schizophyllum commune
2.4.1.231 2.2
-
 alpha-D-glucose 1-phosphate 30°C, pH 6.6, recombinant enzyme Schizophyllum commune
2.4.1.231 35
-
 alpha-D-glucose 30°C, pH 6.6, natural enzyme Schizophyllum commune
2.4.1.231 46
-
 alpha-D-glucose 30°C, pH 6.6, recombinant enzyme Schizophyllum commune
2.4.1.231 53
-
 alpha,alpha-trehalose 30°C, pH 6.6, recombinant enzyme Schizophyllum commune
2.4.1.231 71
-
 alpha,alpha-trehalose 30°C, pH 6.6, natural enzyme Schizophyllum commune

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.231 Schizophyllum commune Q2HZZ3 BT 2115
-
2.4.1.231 Schizophyllum commune BT 2115 Q2HZZ3 BT 2115
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.231 recombinant enzyme Schizophyllum commune

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.231 alpha-D-glucose + alpha-D-glucose 1-phosphate ratio of phosphorolysis to hydrolysis is 46000 Schizophyllum commune alpha,alpha-trehalose + phosphate
-
 r
2.4.1.231 alpha-D-glucose + alpha-D-glucose 1-phosphate ratio of phosphorolysis to hydrolysis is 46000 Schizophyllum commune BT 2115 alpha,alpha-trehalose + phosphate
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.4.1.231 ScTPase
-
 Schizophyllum commune

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.231 9.5
-
 alpha-D-glucose 1-phosphate 30°C, pH 6.6, natural enzyme Schizophyllum commune
2.4.1.231 9.5
-
 alpha-D-glucose 30°C, pH 6.6, natural enzyme Schizophyllum commune
2.4.1.231 13.3
-
 phosphate 30°C, pH 6.6, natural enzyme Schizophyllum commune
2.4.1.231 13.3
-
 alpha,alpha-trehalose 30°C, pH 6.6, natural enzyme Schizophyllum commune
2.4.1.231 14.1
-
 alpha-D-glucose 1-phosphate 30°C, pH 6.6, recombinant enzyme Schizophyllum commune
2.4.1.231 14.1
-
 alpha-D-glucose 30°C, pH 6.6, recombinant enzyme Schizophyllum commune
2.4.1.231 16.4
-
 phosphate 30°C, pH 6.6, recombinant enzyme Schizophyllum commune
2.4.1.231 16.4
-
 alpha,alpha-trehalose 30°C, pH 6.6, recombinant enzyme Schizophyllum commune

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.231 0.0017
-
 validoxylamine A
-
 Schizophyllum commune
2.4.1.231 5.8
-
 alpha-D-glucose 1-phosphate 30°C, pH 6.6, natural enzyme Schizophyllum commune
2.4.1.231 5.9
-
 alpha-D-glucose 1-phosphate 30°C, pH 6.6, recombinant enzyme Schizophyllum commune
2.4.1.231 12
-
 phosphate 30°C, pH 6.6, recombinant enzyme Schizophyllum commune
2.4.1.231 26
-
 phosphate 30°C, pH 6.6, natural enzyme Schizophyllum commune