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Literature summary extracted from

  • Han, Q.; Kim, S.R.; Ding, H.; Li, J.
    Evolution of two alanine glyoxylate aminotransferases in mosquito (2006), Biochem. J., 397, 473-481.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.44 expressed in Sf9 cells Aedes aegypti

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.44 2.6
-
 L-alanine in 100 mM potassium phosphate buffer (pH 7.5) for 5 min at 45°C Aedes aegypti
2.6.1.44 3.7
-
 kynurenine in 100 mM potassium phosphate buffer (pH 7.5) for 5 min at 45°C Aedes aegypti
2.6.1.44 18
-
 3-hydroxykynurenine in 100 mM potassium phosphate buffer (pH 7.5) for 5 min at 45°C Aedes aegypti
2.6.1.44 34
-
 L-alanine in 100 mM potassium phosphate buffer (pH 7.5) for 5 min at 45°C Aedes aegypti

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.44 Aedes aegypti Q3LSM4 Yellowfever mosquito
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.44 DEAE-Sepharose chromatography, phenyl-Sepharose chromatography, hydroxyapatite chromatography, and gel filtration Aedes aegypti

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.44 pupa
-
 Aedes aegypti
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.44 3-hydroxykynurenine + glyoxylate
-
 Aedes aegypti xanthurenic acid + glycine
-
 ?
2.6.1.44 kynurenine + glyoxylate
-
 Aedes aegypti 4-(2-aminophenyl)-2,4-dioxobutanoate + glycine
-
 ?
2.6.1.44 L-alanine + glyoxylate
-
 Aedes aegypti pyruvate + glycine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.6.1.44 3-hydroxykynurenine transaminase
-
 Aedes aegypti
2.6.1.44 AGT
-
 Aedes aegypti
2.6.1.44 alanine glyoxylate aminotransferase
-
 Aedes aegypti

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.6.1.44 80
-
 high activity even at 80°C Aedes aegypti

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.44 80
-
 high activity even at 80°C Aedes aegypti

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.44 12
-
 kynurenine in 100 mM potassium phosphate buffer (pH 7.5) for 5 min at 45°C Aedes aegypti
2.6.1.44 20
-
 3-hydroxykynurenine in 100 mM potassium phosphate buffer (pH 7.5) for 5 min at 45°C Aedes aegypti
2.6.1.44 34.2
-
 L-alanine in 100 mM potassium phosphate buffer (pH 7.5) for 5 min at 45°C Aedes aegypti
2.6.1.44 117
-
 L-alanine in 100 mM potassium phosphate buffer (pH 7.5) for 5 min at 45°C Aedes aegypti

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.44 9
-
-
 Aedes aegypti

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.44 7 10 high activity levels are noted at pH 7.0-10.0 Aedes aegypti

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.6.1.44 6
-
 shows little activity at pH 6.0 Aedes aegypti
2.6.1.44 7 10 high activity levels are noted at pH 7.0-10.0 Aedes aegypti

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.44 pyridoxal 5'-phosphate 0.04 mM Aedes aegypti