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Literature summary extracted from

  • Law, G.H.; Gandelman, O.A.; Tisi, L.C.; Lowe, C.R.; Murray, J.A.
    Mutagenesis of solvent-exposed amino acids in Photinus pyralis luciferase improves thermostability and pH-tolerance (2006), Biochem. J., 397, 305-312.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.12.7 expressed in Escherichia coli BL21(DE3)pLysS cells Photinus pyralis

Protein Variants

EC Number Protein Variants Comment Organism
1.13.12.7 F14R improved pH tolerance and stability up to 45°C, no decrease in specific activity relative to the recombinant wild type enzyme Photinus pyralis
1.13.12.7 F14R/L35Q/V182K/I232K/F465R improved pH tolerance and stability up to 45°C, no decrease in specific activity relative to the recombinant wild type enzyme Photinus pyralis
1.13.12.7 F465R improved pH tolerance and stability up to 45°C, no decrease in specific activity relative to the recombinant wild type enzyme Photinus pyralis
1.13.12.7 I232K improved pH tolerance and stability up to 45°C, no decrease in specific activity relative to the recombinant wild type enzyme Photinus pyralis
1.13.12.7 L35Q improved pH tolerance and stability up to 45°C, no decrease in specific activity relative to the recombinant wild type enzyme Photinus pyralis
1.13.12.7 V182K improved pH tolerance and stability up to 45°C, no decrease in specific activity relative to the recombinant wild type enzyme Photinus pyralis

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.7 Photinus pyralis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.12.7 Ni2+-nitrilotriacetate agarose column chromatography Photinus pyralis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.7 Photinus luciferin + O2 + ATP
-
 Photinus pyralis oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.12.7 Photinus pyralis luciferase
-
 Photinus pyralis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.12.7 43
-
-
 Photinus pyralis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.13.12.7 37 45
-
 Photinus pyralis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.12.7 45
-
 mutant enzymes F14R, L35Q, V182K, I232K and F465R are stable up to 45°C Photinus pyralis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.12.7 7.8
-
-
 Photinus pyralis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.13.12.7 Photinus pyralis His10-tagged wild type enzyme
-
 7.2