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Literature summary extracted from

  • Yoshikane, Y.; Yokochi, N.; Ohnishi, K.; Hayashi, H.; Yagi, T.
    Molecular cloning, expression and characterization of pyridoxamine-pyruvate aminotransferase (2006), Biochem. J., 396, 499-507.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.30 expressed in Escherichia coli strains JM109 and BL21(DE3) Mesorhizobium loti
2.6.1.30 expression in Escherichia coli Mesorhizobium loti

Protein Variants

EC Number Protein Variants Comment Organism
2.6.1.30 C198A enzyme shows the same specific activity as that of the wild type enzyme Mesorhizobium loti
2.6.1.30 C198A shows activity of the wild type enzyme Mesorhizobium loti
2.6.1.30 K197L mutant enzyme shows no activity Mesorhizobium loti
2.6.1.30 K197L shows no activity Mesorhizobium loti

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.30 additional information hydroxylamine (10 mM), phenylhydrazine (1 mM) and sodium borohydride (1 mM) do not inactivate the enzyme Mesorhizobium loti

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.30 0.044
-
 pyridoxamine
-
 Mesorhizobium loti
2.6.1.30 0.044
-
 pyridoxamine 30°C, pH 9.0 Mesorhizobium loti
2.6.1.30 0.059
-
 pyridoxal
-
 Mesorhizobium loti
2.6.1.30 0.059
-
 pyridoxal 30°C, pH 9.0 Mesorhizobium loti
2.6.1.30 0.34
-
 pyruvate
-
 Mesorhizobium loti
2.6.1.30 0.34
-
 pyruvate 30°C, pH 9.0 Mesorhizobium loti
2.6.1.30 7.1
-
 2-oxobutyrate
-
 Mesorhizobium loti
2.6.1.30 7.1
-
 2-oxobutyrate 30°C, pH 9.0 Mesorhizobium loti
2.6.1.30 11
-
 L-alanine
-
 Mesorhizobium loti
2.6.1.30 11
-
 L-alanine 30°C, pH 9.0 Mesorhizobium loti
2.6.1.30 20
-
 (S)-2-aminobutyrate
-
 Mesorhizobium loti
2.6.1.30 20
-
 (S)-2-aminobutyrate 30°C, pH 9.0 Mesorhizobium loti

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.30 41000
-
 4 * 41000, SDS-PAGE Mesorhizobium loti
2.6.1.30 41589
-
 4 * 41589, calculated from amino acid sequence Mesorhizobium loti
2.6.1.30 41589
-
 4 * 41589, calculated from sequence Mesorhizobium loti
2.6.1.30 154000
-
 gel filtration Mesorhizobium loti

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.30 Mesorhizobium loti
-
-
-
2.6.1.30 Mesorhizobium loti Q988B8
-
-
2.6.1.30 Mesorhizobium loti MAFF303099
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.30 partial Mesorhizobium loti
2.6.1.30 partial, QA52 column chromatography and butyl-Toyopearl column chromatography Mesorhizobium loti

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.30 (S)-2-aminobutyrate + pyruvate (S)-2-aminobutyrate is a poor substrate Mesorhizobium loti ?
-
 ?
2.6.1.30 (S)-2-aminobutyrate + pyruvate (S)-2-aminobutyrate is a poor substrate Mesorhizobium loti MAFF303099 ?
-
 ?
2.6.1.30 additional information the enzyme shows less than 0.5% of the activity of pyridoxamine towards pyridoxamine 5'-phosphate (3.3 mM) with pyruvate as the amino acceptor, the enzyme shows less than 0.5% of the activity of pyruvate towards 2-oxoglutarate, 3-hydroxypyruvate, indol-pyruvate, phenylpyruvate, 3-methyl-2-oxobutyrate and 4-hydroxyphenylpyruvate when they are used at 10 mM Mesorhizobium loti ?
-
 ?
2.6.1.30 additional information the enzyme shows less than 0.5% of the activity of pyridoxamine towards pyridoxamine 5'-phosphate (3.3 mM) with pyruvate as the amino acceptor, the enzyme shows less than 0.5% of the activity of pyruvate towards 2-oxoglutarate, 3-hydroxypyruvate, indol-pyruvate, phenylpyruvate, 3-methyl-2-oxobutyrate and 4-hydroxyphenylpyruvate when they are used at 10 mM Mesorhizobium loti MAFF303099 ?
-
 ?
2.6.1.30 pyridoxamine + 2-oxobutyrate
-
 Mesorhizobium loti ?
-
 ?
2.6.1.30 pyridoxamine + 2-oxobutyrate
-
 Mesorhizobium loti MAFF303099 ?
-
 ?
2.6.1.30 pyridoxamine + 2-oxobutyrate poor substrate Mesorhizobium loti pyridoxal + (S)-2-aminobutyrate
-
 r
2.6.1.30 pyridoxamine + pyruvate
-
 Mesorhizobium loti pyridoxal + L-alanine
-
 ?
2.6.1.30 pyridoxamine + pyruvate
-
 Mesorhizobium loti pyridoxal + L-alanine
-
 r
2.6.1.30 pyridoxamine + pyruvate
-
 Mesorhizobium loti MAFF303099 pyridoxal + L-alanine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.30 homotetramer 4 * 41000, SDS-PAGE Mesorhizobium loti
2.6.1.30 homotetramer 4 * 41589, calculated from amino acid sequence Mesorhizobium loti
2.6.1.30 tetramer 4 * 41000, SDS-PAGE Mesorhizobium loti
2.6.1.30 tetramer 4 * 41589, calculated from sequence Mesorhizobium loti

Synonyms

EC Number Synonyms Comment Organism
2.6.1.30 PPAT
-
 Mesorhizobium loti
2.6.1.30 pyridoxamine-pyruvate aminotransferase
-
 Mesorhizobium loti

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.30 70
-
 at pH 9, 100 mM borate/KOH buffer Mesorhizobium loti
2.6.1.30 70
-
 in 100 mM borate/KOH buffer pH 9.0 Mesorhizobium loti

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.6.1.30 30 80 30: 17% of maximal activity, 80°C: 3% of maximal activity Mesorhizobium loti

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.30 30 80 17% and 3% of the maximal activity is observed at 30°C and 80°C, respectively Mesorhizobium loti

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.30 2 8 pyridoxamine
-
 Mesorhizobium loti
2.6.1.30 2 8 pyridoxamine 30°C, pH 9.0 Mesorhizobium loti
2.6.1.30 9
-
 (S)-2-aminobutyrate
-
 Mesorhizobium loti
2.6.1.30 9
-
 (S)-2-aminobutyrate 30°C, pH 9.0 Mesorhizobium loti
2.6.1.30 24
-
 2-oxobutyrate
-
 Mesorhizobium loti
2.6.1.30 24
-
 2-oxobutyrate 30°C, pH 9.0 Mesorhizobium loti
2.6.1.30 29
-
 pyruvate
-
 Mesorhizobium loti
2.6.1.30 29
-
 pyruvate 30°C, pH 9.0 Mesorhizobium loti
2.6.1.30 41
-
 L-alanine
-
 Mesorhizobium loti
2.6.1.30 41
-
 pyridoxal
-
 Mesorhizobium loti
2.6.1.30 41
-
 L-alanine 30°C, pH 9.0 Mesorhizobium loti
2.6.1.30 41
-
 pyridoxal 30°C, pH 9.0 Mesorhizobium loti

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.30 9.5
-
 estimated based on the Km and kcat values Mesorhizobium loti

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.30 additional information pyridoxal 5'-phosphate-independent enzyme Mesorhizobium loti