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Literature summary extracted from

  • Panethymitaki, C.; Bowyer, P.W.; Price, H.P.; Leatherbarrow, R.J.; Brown, K.A.; Smith, D.F.
    Characterization and selective inhibition of myristoyl-CoA:protein N-myristoyltransferase from Trypanosoma brucei and Leishmania major (2006), Biochem. J., 396, 277-285.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.97 expression in Escherichia coli Trypanosoma brucei

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.97 CP-014553
-
 Leishmania major
2.3.1.97 CP-014553 IC50: 460 nM Trypanosoma brucei
2.3.1.97 CP-030890-27
-
 Leishmania major
2.3.1.97 CP-030890-27 IC50: 250 nM Trypanosoma brucei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.97 0.00137
-
 myristoyl-CoA
-
 Leishmania major
2.3.1.97 0.00178
-
 myristoyl-CoA
-
 Trypanosoma brucei
2.3.1.97 0.0113
-
 GCGGSKVK
-
 Trypanosoma brucei
2.3.1.97 0.11
-
 GQLFTSLN
-
 Leishmania major

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.97 50500
-
 x * 50500, SDS-PAGE Trypanosoma brucei

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.97 Leishmania major Q9GPZ4
-
-
2.3.1.97 Trypanosoma brucei Q388H8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.97
-
 Trypanosoma brucei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.97 myristoyl-CoA + GCGGSKVK
-
 Trypanosoma brucei N-myristoylglycyl-CGGSKVK + CoA
-
 ?
2.3.1.97 myristoyl-CoA + GQLFTSLN
-
 Leishmania major N-myristoylglycyl-GQLFTSLN + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.97 ? x * 50500, SDS-PAGE Trypanosoma brucei

Synonyms

EC Number Synonyms Comment Organism
2.3.1.97 LmNMT
-
 Leishmania major
2.3.1.97 myristoyl-CoA:protein N-myristoyltransferase
-
 Leishmania major
2.3.1.97 myristoyl-CoA:protein N-myristoyltransferase
-
 Trypanosoma brucei
2.3.1.97 TbNMT
-
 Trypanosoma brucei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.97 18.2
-
 GCGGSKVK
-
 Trypanosoma brucei
2.3.1.97 23.6
-
 myristoyl-CoA
-
 Leishmania major
2.3.1.97 24
-
 GQLFTSLN
-
 Leishmania major
2.3.1.97 76.8
-
 myristoyl-CoA
-
 Trypanosoma brucei

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.3.1.97 0.00025
-
 IC50: 250 nM Trypanosoma brucei CP-030890-27
2.3.1.97 0.00046
-
 IC50: 460 nM Trypanosoma brucei CP-014553