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Literature summary extracted from

  • Graham, C.E.; Brocklehurst, K.; Pickersgill, R.W.; Warren, M.J.
    Characterization of retinaldehyde dehydrogenase 3 (2006), Biochem. J., 394, 67-75.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.36 into the cloning vector pGEM-T Easy and subcloned into the expression vector pET14b Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.36 F471L mutant Mus musculus
1.2.1.36 T1411C mutant Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.36 3,3',5-tri-iodothyronine
-
 Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.36 0.0002
-
 all-trans-retinal wild-type Mus musculus
1.2.1.36 0.0002
-
 all-trans-retinal F471L mutant Mus musculus
1.2.1.36 0.0007
-
 octanal wild-type Mus musculus
1.2.1.36 0.0009
-
 octanal F471L mutant Mus musculus
1.2.1.36 0.0065
-
 decanal wild-type Mus musculus
1.2.1.36 0.0221
-
 hexanal wild-type Mus musculus
1.2.1.36 0.0431
-
 NAD+ F471L mutant Mus musculus
1.2.1.36 0.0528
-
 NAD+ wild-type Mus musculus
1.2.1.36 0.103
-
 benzaldehyde wild-type Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.36 retinal + NAD+ + H2O Mus musculus
-
 retinoate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.36 Mus musculus Q9JHW9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.36 using a nickel-chelating Sepharose and a S300 HR gel-filtration column Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.36 benzaldehyde + NAD+ + H2O
-
 Mus musculus benzoate + NADH + H+
-
 ?
1.2.1.36 decanal + NAD+ + H2O
-
 Mus musculus decanoate + NADH + H+
-
 ?
1.2.1.36 hexanal + NAD+ + H2O
-
 Mus musculus hexanoate + NADH + H+
-
 ?
1.2.1.36 octanal + NAD+ + H2O
-
 Mus musculus octanoate + NADH + H+
-
 ?
1.2.1.36 retinal + NAD+ + H2O
-
 Mus musculus retinoate + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.36 RALDH3
-
 Mus musculus
1.2.1.36 Retinal dehydrogenase
-
 Mus musculus
1.2.1.36 retinaldehyde dehydrogenase 3
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.36 37
-
 activity assay Mus musculus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.36 0.2
-
 benzaldehyde wild-type Mus musculus
1.2.1.36 0.3
-
 hexanal wild-type Mus musculus
1.2.1.36 0.4
-
 decanal wild-type Mus musculus
1.2.1.36 3.1
-
 NAD+ F471L mutant Mus musculus
1.2.1.36 3.3
-
 NAD+ wild-type Mus musculus
1.2.1.36 3.9
-
 retinal all-trans-retinal, wild-type Mus musculus
1.2.1.36 4.8
-
 octanal wild-type Mus musculus
1.2.1.36 5.4
-
 octanal F471L mutant Mus musculus
1.2.1.36 13
-
 retinal all-trans-retinal, F471L mutant Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.36 8.5
-
 activity assay Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.36 NADH
-
 Mus musculus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.2.1.36 0.0377
-
 3,3',5-tri-iodothyronine
-
 Mus musculus