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Literature summary extracted from

  • Datta, R.; Das, I.; Sen, B.; Chakraborty, A.; Adak, S.; Mandal, C.; Datta, A.K.
    Homology-model-guided site-specific mutagenesis reveals the mechanisms of substrate binding and product-regulation of adenosine kinase from Leishmania donovani (2006), Biochem. J., 394, 35-42.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.20 overexpression in Escherichia coli Leishmania donovani

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.20 D16E specific activity at the highest attainable adenosine concentration is approximately 2.5-fold lower than that of the wild-type enzyme. Non-saturation kinetics of the D16E mutant are indicative of weaker binding Leishmania donovani
2.7.1.20 D16N kcat/Km value for adenosine is lowered more than 1000fold. Mutation does not significantly affected the apparent Km for ATP Leishmania donovani
2.7.1.20 D16V kcat/Km value for adenosine is lowered more than 1000fold. Mutation does not significantly affected the apparent Km for ATP Leishmania donovani
2.7.1.20 R131A mutant shows abrogated AMP-binding, insensitivity towards AMP inhibition, unaltered Km-value for adenosine Leishmania donovani

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.20 2'-deoxyadenosine
-
 Leishmania donovani
2.7.1.20 3'-deoxyadenosine
-
 Leishmania donovani
2.7.1.20 AMP inhibits both D16V and D16N mutant enzymes to similar extents to that observed with the wild-type enzyme, displays a much reduced ability in inhibiting R131A mutant enzyme Leishmania donovani

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.20 0.005
-
 adenosine wild-type enzyme Leishmania donovani
2.7.1.20 0.0059
-
 adenosine mutant enzyme R131A Leishmania donovani
2.7.1.20 0.017
-
 ATP mutant enzyme D16N Leishmania donovani
2.7.1.20 0.02
-
 ATP mutant enzyme D16V Leishmania donovani
2.7.1.20 0.036
-
 ATP wild-type enzyme Leishmania donovani
2.7.1.20 0.036
-
 3'-deoxyadenosine wild-type enzyme Leishmania donovani
2.7.1.20 0.05
-
 ATP mutant enzyme R131A Leishmania donovani
2.7.1.20 0.077
-
 2'-deoxyadenosine wild-type enzyme Leishmania donovani
2.7.1.20 1.293
-
 adenosine mutant enzyme D16V Leishmania donovani
2.7.1.20 1.575
-
 adenosine mutant enzyme D16N Leishmania donovani

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.20 Leishmania donovani
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.20 ATP + 2'-deoxy-adenosine
-
 Leishmania donovani ADP + 2'-deoxy-AMP
-
 ?
2.7.1.20 ATP + adenosine
-
 Leishmania donovani ADP + AMP
-
 ?
2.7.1.20 ATP + cordycepin
-
 Leishmania donovani ADP + cordycepin 5'-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.20 LdAdK
-
 Leishmania donovani

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.20 0.733
-
 ATP mutant enzyme R131A Leishmania donovani
2.7.1.20 0.733
-
 adenosine mutant enzyme R131A Leishmania donovani
2.7.1.20 5.1
-
 ATP mutant enzyme D16V Leishmania donovani
2.7.1.20 5.1
-
 adenosine mutant enzyme D16V Leishmania donovani
2.7.1.20 5.2
-
 ATP mutant enzyme D16N Leishmania donovani
2.7.1.20 5.2
-
 adenosine mutant enzyme D16N Leishmania donovani
2.7.1.20 5.25
-
 2'-deoxyadenosine wild-type enzyme Leishmania donovani
2.7.1.20 6.7
-
 3'-deoxyadenosine wild-type enzyme Leishmania donovani
2.7.1.20 18
-
 ATP wild-type enzyme Leishmania donovani
2.7.1.20 18
-
 adenosine wild-type enzyme Leishmania donovani
2.7.1.20 24.15
-
 ATP wild-type enzyme Leishmania donovani
2.7.1.20 24.15
-
 adenosine wild-type enzyme Leishmania donovani

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.20 0.0033
-
 3'-deoxyadenosine wild-type enzyme Leishmania donovani
2.7.1.20 0.01575
-
 2'-deoxyadenosine wild-type enzyme Leishmania donovani