Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Khajamohiddin, S.; Babu, P.S.; Chakka, D.; Merrick, M.; Bhaduri, A.; Sowdhamini, R.; Siddavattam, D.
    A novel meta-cleavage product hydrolase from Flavobacterium sp. ATCC27551 (2006), Biochem. Biophys. Res. Commun., 351, 675-681.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.7.1.9 additional information EDTA has no effect Flavobacterium sp.

Application

EC Number Application Comment Organism
3.7.1.9 molecular biology orf234, encoding an alpha/beta hydrolase, which is distantly related to the meta-fission product hydrolases such as XylF, PhnD, and CumD Flavobacterium sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.7.1.9 overexpression as an N-terminal His-tag from pSM12 in Escherichia coli BL21(DE3), or from plasmid pSM13 in Pseudomonas aeruginosa PAO1161 Flavobacterium sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.7.1.9 DEPC inhibits by 82% Flavobacterium sp.
3.7.1.9 additional information N-ethylmaleimide and p-chloromercuribenzoate show no significant effect Flavobacterium sp.
3.7.1.9 PMSF inhibits by 75% Flavobacterium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.7.1.9 0.0048
-
 2-hydroxy-6-oxohepta-2,4-dienoate
-
 Flavobacterium sp.
3.7.1.9 0.0057
-
 2-hydroxymuconic semialdehyde
-
 Flavobacterium sp.
3.7.1.9 0.0074
-
 2-hydroxy-5-methylmuconic semialdehyde
-
 Flavobacterium sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.7.1.9 additional information Fe2+, Ca2+, Cu2+, Mg2+, Mn2+, Co2+, Li2+, and Zn2+ have no effect Flavobacterium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.7.1.9 30000
-
 4 * 30000, SDS-PAGE Flavobacterium sp.
3.7.1.9 120000
-
 gel filtration Flavobacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
3.7.1.9 Flavobacterium sp.
-
 ATCC27551
-
3.7.1.9 Flavobacterium sp. ATCC 27551
-
 ATCC27551
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.7.1.9 by affinity chromatography Flavobacterium sp.

Storage Stability

EC Number Storage Stability Organism
3.7.1.9 -20°C, 20% glycerol, 15 days Flavobacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.7.1.9 2-hydroxy-5-methylmuconic semialdehyde + H2O
-
 Flavobacterium sp. ?
-
 ?
3.7.1.9 2-hydroxy-5-methylmuconic semialdehyde + H2O
-
 Flavobacterium sp. ATCC 27551 ?
-
 ?
3.7.1.9 2-hydroxy-6-oxohepta-2,4-dienoate + H2O
-
 Flavobacterium sp. ?
-
 ?
3.7.1.9 2-hydroxy-6-oxohepta-2,4-dienoate + H2O
-
 Flavobacterium sp. ATCC 27551 ?
-
 ?
3.7.1.9 2-hydroxymuconic semialdehyde + H2O
-
 Flavobacterium sp. ?
-
 ?
3.7.1.9 2-hydroxymuconic semialdehyde + H2O
-
 Flavobacterium sp. ATCC 27551 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.7.1.9 homotetramer 4 * 30000, SDS-PAGE Flavobacterium sp.

Synonyms

EC Number Synonyms Comment Organism
3.7.1.9 meta-cleavage product hydrolase
-
 Flavobacterium sp.
3.7.1.9 MfhA
-
 Flavobacterium sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.7.1.9 75
-
 loses just 20% activity after incubation for 2 hours Flavobacterium sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.7.1.9 5 85 above 75°C enzyme activity declines rapidly Flavobacterium sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.7.1.9 16
-
 2-hydroxy-5-methylmuconic semialdehyde
-
 Flavobacterium sp.
3.7.1.9 42
-
 2-hydroxymuconic semialdehyde
-
 Flavobacterium sp.
3.7.1.9 57
-
 2-hydroxy-6-oxohepta-2,4-dienoate
-
 Flavobacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.7.1.9 8.5
-
-
 Flavobacterium sp.