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Literature summary extracted from

  • Assem, F.L.; Kirk, C.J.; Chipman, J.K.
    Substrate characterisation of a recombinant sulfotransferase SULT1 and mRNA expression in chub (Leuciscus cephalus) tissues (2006), Biochem. Biophys. Res. Commun., 349, 900-905.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.2.1 SULT1,3, cloning from liver, DNA and amino acid sequence determination and analysis, including the promoter region, expression in Escherichia coli strain BL21(DE3) Squalius cephalus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.1 0.0017
-
 genistein pH 7.0, 20°C, recombinant SULT1,3 Squalius cephalus
2.8.2.1 0.0044
-
 daidzein pH 7.0, 20°C, recombinant SULT1,3 Squalius cephalus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.1 35000
-
 x * 35000, recombinant SULT1,3, SDS-PAGE Squalius cephalus

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.1 Squalius cephalus A0FIP8
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.1 brain low SULT1,3 expression level Squalius cephalus
-
2.8.2.1 gill low SULT1,3 expression level Squalius cephalus
-
2.8.2.1 gonad low SULT1,3 expression level Squalius cephalus
-
2.8.2.1 kidney high SULT1,3 expression level Squalius cephalus
-
2.8.2.1 liver high SULT1,3 expression level, mature male fish display higher hepatic SULT1,3 mRNA expression compared to female fish Squalius cephalus
-
2.8.2.1 additional information quantitative tissue expression analysis of SULT1,3, overview Squalius cephalus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.1 3'-phosphoadenylyl sulfate + 4-nitrophenol
-
 Squalius cephalus adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
-
 ?
2.8.2.1 3'-phosphoadenylyl sulfate + daidzein
-
 Squalius cephalus adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.1 3'-phosphoadenylyl sulfate + dopamine low activity Squalius cephalus adenosine 3',5'-bisphosphate + dopamine sulfate
-
 ?
2.8.2.1 3'-phosphoadenylyl sulfate + genistein
-
 Squalius cephalus adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.1 3'-phosphoadenylyl sulfate + nonyl phenol low activity Squalius cephalus adenosine 3',5'-bisphosphate + nonyl phenyl sulfate
-
 ?
2.8.2.1 3'-phosphoadenylyl sulfate + octyl phenol low activity Squalius cephalus adenosine 3',5'-bisphosphate + octyl phenyl sulfate
-
 ?
2.8.2.1 additional information no activity with the synthetic estrogens ethynyl estradiol and diethylstilbestrol or endogenous substrates estrone, 17beta-estradiol, dehydroepiandrosterone, and the thyroid hormones 3,3',5-triiodo-L-thyronine T3 and thyronine T4, the enzyme contains two conserved co-substrate binding motifs for 30-phosphoadenosine 50-phosphosulfate: RKGxxGDWKxxFT and YPKSGTxW Squalius cephalus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.2.1 ? x * 35000, recombinant SULT1,3, SDS-PAGE Squalius cephalus
2.8.2.1 More the enzyme contains two conserved co-substrate binding motifs for 30-phosphoadenosine 50-phosphosulfate: RKGxxGDWKxxFT and YPKSGTxW Squalius cephalus

Synonyms

EC Number Synonyms Comment Organism
2.8.2.1 sulfotransferase 1,3
-
 Squalius cephalus
2.8.2.1 SULT1,3
-
 Squalius cephalus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.2.1 20
-
 assay at Squalius cephalus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.1 7
-
 assay at Squalius cephalus