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Literature summary extracted from

  • Hurley, T.D.; Walls, C.; Bennett, J.R.; Roach, P.J.; Wang, M.
    Direct detection of glycogenin reaction products during glycogen initiation (2006), Biochem. Biophys. Res. Commun., 348, 374-378.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.186 expresssion of recombinant protein in the CGSC Escherichia coli cell line 4997, which lacks UDP-glucose pyrophosphorylase activity Oryctolagus cuniculus

General Stability

EC Number General Stability Organism
2.4.1.186 concentrated to 8 mg/ml, and 100 microl aliquots are flash frozen in liquid nitrogen and stored at -80°C. Glycogenin stored in this manner is stable for at least 2 years. Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.186 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.186 nickel-NTA column and Q-Sepharose column Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.186 muscle
-
 Oryctolagus cuniculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.186 UDP-alpha-D-glucose + glycogenin
-
 Oryctolagus cuniculus additional information after 60 min of incubation the glycogenin molecules possess an average glucosyl chain length of 11.3 residues ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.186 additional information
-
 additional information the catalytic effectiveness of glycogenin falls off dramatically as the average glucosyl chain length increases Oryctolagus cuniculus
2.4.1.186 0.0053
-
 UDP-glucose glucosylated protein, 14C-glucose incorporation assay Oryctolagus cuniculus
2.4.1.186 0.0083
-
 UDP-glucose glucosylated protein, 4-10 interval, mass sprectrometry assay Oryctolagus cuniculus
2.4.1.186 0.0083
-
 UDP-glucose non-glucosylated protein, 14C-glucose incorporation assay Oryctolagus cuniculus
2.4.1.186 0.0183
-
 UDP-glucose non-glucosylated protein, mass sprectrometry assay Oryctolagus cuniculus