EC Number | Cloned (Comment) | Organism |
---|---|---|
6.3.4.15 | expression in Escherichia coli BL21(DE3) | Sulfurisphaera tokodaii |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
6.3.4.15 | A43G | catalytic efficiency is 2fold lower than catalytic efficiency of wild-type enzyme | Sulfurisphaera tokodaii |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.3.4.15 | ATP | - |
Sulfurisphaera tokodaii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.4.15 | 0.000026 | - |
biotin | pH 8.0, 37°C, mutant enzyme A43G | Sulfurisphaera tokodaii | |
6.3.4.15 | 0.00044 | - |
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein]-lysine | pH 8.0, 70°C, wild type enzyme | Sulfurisphaera tokodaii | |
6.3.4.15 | 0.00058 | - |
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein]-lysine | pH 8.0, 70°C, mutant enzyme A43G | Sulfurisphaera tokodaii | |
6.3.4.15 | 0.018 | - |
ATP | pH 8.0, 37°C, mutant enzyme A43G | Sulfurisphaera tokodaii | |
6.3.4.15 | 0.063 | - |
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine | pH 8.0, 70°C, mutant enzyme A43G | Sulfurisphaera tokodaii | |
6.3.4.15 | 0.101 | - |
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine | pH 8.0, 70°C, wild type enzyme | Sulfurisphaera tokodaii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.3.4.15 | Sulfurisphaera tokodaii | F9VNG7 | - |
- |
6.3.4.15 | Sulfurisphaera tokodaii 7 | F9VNG7 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.3.4.15 | - |
Sulfurisphaera tokodaii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.4.15 | ATP + biotin + [Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine | the enzyme from Sulfolobus tokodaii cannot use wild-type biotin carboxyl carrier protein from Escherichia coli as a substrate | Sulfurisphaera tokodaii | AMP + diphosphate + [Sulfolobus tokodaii biotin-carboxyl-carrier-protein mutant S136M]-biotinyllysine | - |
? | |
6.3.4.15 | ATP + biotin + [Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine | the enzyme from Sulfolobus tokodaii cannot use wild-type biotin carboxyl carrier protein from Escherichia coli as a substrate | Sulfurisphaera tokodaii 7 | AMP + diphosphate + [Sulfolobus tokodaii biotin-carboxyl-carrier-protein mutant S136M]-biotinyllysine | - |
? | |
6.3.4.15 | ATP + biotin + [Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein]-lysine | the enzyme from Sulfolobus tokodaii cannot use wild-type biotin carboxyl carrier protein from Escherichia coli as a substrate | Sulfurisphaera tokodaii | AMP + diphosphate + [Sulfolobus tokodaii biotin-carboxyl-carrier-protein]-biotinyllysine | - |
? | |
6.3.4.15 | ATP + biotin + [Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein]-lysine | the enzyme from Sulfolobus tokodaii cannot use wild-type biotin carboxyl carrier protein from Escherichia coli as a substrate | Sulfurisphaera tokodaii 7 | AMP + diphosphate + [Sulfolobus tokodaii biotin-carboxyl-carrier-protein]-biotinyllysine | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.3.4.15 | More | biotin-protein-ligase/holo-biotin-carboxyl-carrier-protein complex | Sulfurisphaera tokodaii |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.3.4.15 | STK_15250 | locus name | Sulfurisphaera tokodaii |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.3.4.15 | 80 | - |
no denaturation | Sulfurisphaera tokodaii |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.4.15 | 0.0022 | - |
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein]-lysine | pH 8.0, 70°C, mutant enzyme A43G | Sulfurisphaera tokodaii | |
6.3.4.15 | 0.004 | - |
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein]-lysine | pH 8.0, 70°C, wild type enzyme | Sulfurisphaera tokodaii | |
6.3.4.15 | 0.082 | - |
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine | pH 8.0, 70°C, mutant enzyme A43G | Sulfurisphaera tokodaii | |
6.3.4.15 | 0.165 | - |
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine | pH 8.0, 70°C, wild type enzyme | Sulfurisphaera tokodaii |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.4.15 | 0.36 | - |
ATP | pH 8.0, 37°C, mutant enzyme A43G | Sulfurisphaera tokodaii | |
6.3.4.15 | 0.62 | - |
ATP | pH 8.0, 37°C, wild type enzyme | Sulfurisphaera tokodaii |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.4.15 | 1.3 | - |
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine | pH 8.0, 70°C, mutant enzyme A43G | Sulfurisphaera tokodaii | |
6.3.4.15 | 1.6 | - |
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine | pH 8.0, 70°C, wild type enzyme | Sulfurisphaera tokodaii | |
6.3.4.15 | 3.8 | - |
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein]-lysine | pH 8.0, 70°C, mutant enzyme A43G | Sulfurisphaera tokodaii | |
6.3.4.15 | 9.1 | - |
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein]-lysine | pH 8.0, 70°C,wild type enzyme | Sulfurisphaera tokodaii |