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Literature summary extracted from

  • Hasegawa, H.; Ichiyama, A.
    Distinctive iron requirement of tryptophan 5-monooxygenase: TPH1 requires dissociable ferrous iron (2005), Biochem. Biophys. Res. Commun., 338, 277-284.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.16.4 2-mercaptoethanol 30 mM, 79.3% activity compared to activation with dithiothreitol Mus musculus
1.14.16.4 2-mercaptoethylamine 30 mM, 93.8% activity compared to activation with dithiothreitol Mus musculus
1.14.16.4 2-mercaptopropionate 30 mM, 11.7% activity compared to activation with dithiothreitol Mus musculus
1.14.16.4 3-Mercaptopropionate 30 mM, 22% activity compared to activation with dithiothreitol Mus musculus
1.14.16.4 dithiothreitol 10 mM Mus musculus
1.14.16.4 glutathione 30 mM, 16.1% activity compared to activation with dithiothreitol Mus musculus
1.14.16.4 L-cysteine 30 mM, 79.5% activity compared to activation with dithiothreitol Mus musculus
1.14.16.4 N-acetylcysteine 30 mM, 31.2% activity compared to activation with dithiothreitol Mus musculus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.16.4 expression in Escherichia coli BL21-CodonPlus(DE3)-RIL as His-tag fusion protein Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.14.16.4 delta1-150 deletion of the N-terminal regulatory domain. Increased solubility when expressed in Escherichia coli. Homo sapiens
1.14.16.4 delta1-150/421-444 deletion of the N-terminal regulatory domain and the C-terminal tetramerisation domain. Increased solubility when expressed in Escherichia coli. The mutant protein shows a 2fold increase of the Km for L-tryptophan and a 3fold decrease of the Km for (6R)-L-erythro-5,6,7,8-tetrahydrobiopterin Homo sapiens

General Stability

EC Number General Stability Organism
1.14.16.4 The half-life of the protein is 30 min at 37°C, while the N-terminal and the N- and C-terminal truncated mutants exhibit half-lives of 203 min and 147 min, respectively Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.16.4 0.01789
-
 (6R)-L-erythro-5,6,7,8-tetrahydrobiopterin
-
 Homo sapiens
1.14.16.4 0.02426
-
 L-tryptophan
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.16.4 Fe2+ required for activity Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.16.4 59000
-
 SDS-PAGE Homo sapiens
1.14.16.4 236000
-
 gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.14.16.4 Homo sapiens
-
-
-
1.14.16.4 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.16.4 Ni-affinity chromatography Homo sapiens
1.14.16.4 one chromatography step, 98% homogeneity Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.16.4 cell culture
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.16.4 5
-
-
 Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.16.4 L-tryptophan + (6R)-L-erythro-5,6,7,8-tetrahydrobiopterin + O2
-
 Mus musculus 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin
-
 ?
1.14.16.4 L-tryptophan + (6R)-L-erythro-5,6,7,8-tetrahydrobiopterin + O2
-
 Homo sapiens 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.16.4 tetramer 4 * 59000, gel filtration and SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.14.16.4 TPH1
-
 Mus musculus
1.14.16.4 TPH2
-
 Homo sapiens
1.14.16.4 tryptophan 5-monooxygenase
-
 Mus musculus
1.14.16.4 tryptophan hydroxylase
-
 Mus musculus
1.14.16.4 tryptophan hydroxylase 2
-
 Homo sapiens