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Literature summary extracted from

  • Hao, J.; Balagurumoorthy, P.; Sarilla, S.; Sundaramoorthy, M.
    Cloning, expression, and characterization of sialic acid synthases (2005), Biochem. Biophys. Res. Commun., 338, 1507-1514.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.56 expressed in Escherichia coli BL21(DE3) Gold cells Neisseria meningitidis
2.5.1.57 expressed in Escherichia coli BL21 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.56 C182A no activity Neisseria meningitidis
2.5.1.56 C182S no activity Neisseria meningitidis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.57 Ba2+ 1 mM, 93% decrease of activity Homo sapiens
2.5.1.57 Ca2+ 1 mM, 76% decrease of activity Homo sapiens
2.5.1.57 Co2+ 1 mM, 15% decrease of activity Homo sapiens
2.5.1.57 Cu2+ 1 mM, 68% decrease of activity Homo sapiens
2.5.1.57 EDTA 10 mM, complete inhibition Homo sapiens
2.5.1.57 Fe2+ 1 mM, 79% decrease of activity Homo sapiens
2.5.1.57 Ni2+ 1 mM, 24% decrease of activity Homo sapiens
2.5.1.57 Zn2+ 1 mM, 87% decrease of activity Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.56 11.6
-
N-acetyl-D-mannosamine the reaction mixture contains 1 mM MgCl2 in 50 mM Tris-acetate buffer, pH 7.5, at 37°C Neisseria meningitidis
2.5.1.57 1.04
-
N-acetyl-D-mannosamine 6-phosphate in 50 mM bicine pH 7.5 at 37°C Homo sapiens
2.5.1.57 2.62
-
D-mannose 6-phosphate in 50 mM bicine pH 7.5 at 37°C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.56 Co2+ 1 mM, 271% relative activity compared to Mg2+ Neisseria meningitidis
2.5.1.56 Mg2+ 1 mM, required for 100% activity Neisseria meningitidis
2.5.1.56 Mn2+ 1 mM, 115% relative activity compared to Mg2+ Neisseria meningitidis
2.5.1.57 Cd2+ 1 mM, 30% increase of activity Homo sapiens
2.5.1.57 Mn2+ 1 mM, 15% increase of activity Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.56 38629
-
1 * 38629, calculated from amino acid sequence Neisseria meningitidis
2.5.1.56 38630
-
calculated from amino acid sequence Neisseria meningitidis
2.5.1.56 38649
-
1 * 38649, ESI-MS Neisseria meningitidis
2.5.1.56 38650
-
ESI-MS Neisseria meningitidis
2.5.1.56 39000
-
2 * 39000, SDS-PAGE Neisseria meningitidis
2.5.1.56 78000
-
SDS-PAGE Neisseria meningitidis
2.5.1.57 42360
-
calculated from sequence of cDNA Homo sapiens
2.5.1.57 42360
-
ESI mass spectrometry Homo sapiens
2.5.1.57 100100
-
SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.56 Neisseria meningitidis
-
-
-
2.5.1.57 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.56 Ni2+ affinity column chromatography Neisseria meningitidis
2.5.1.57 Ni2+-Sepharose Fast Flow column chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.56 N-acetyl-D-mannosamine + phosphoenolpyruvate + H2O
-
Neisseria meningitidis N-acetylneuraminate + phosphate
-
?
2.5.1.57 D-mannose 6-phosphate + ?
-
Homo sapiens ?
-
?
2.5.1.57 N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
-
Homo sapiens N-acetyl-neuraminate 9-phosphate + phosphate
-
?

Subunits

EC Number Subunits Comment Organism
2.5.1.56 dimer 2 * 39000, SDS-PAGE Neisseria meningitidis
2.5.1.56 monomer 1 * 38629, calculated from amino acid sequence Neisseria meningitidis
2.5.1.56 monomer 1 * 38649, ESI-MS Neisseria meningitidis
2.5.1.57 dimer or trimer the molecular weight is more than the size of a dimer, but smaller than a trimer Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.5.1.56 NeuNAc synthase
-
Neisseria meningitidis
2.5.1.56 sialic acid synthase
-
Neisseria meningitidis
2.5.1.57 N-acetylneuraminic acid-9-phosphate synthase
-
Homo sapiens
2.5.1.57 NeuNAc-9-P synthase
-
Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.56 37
-
-
Neisseria meningitidis
2.5.1.57 37
-
-
Homo sapiens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.5.1.56 10 70
-
Neisseria meningitidis
2.5.1.57 10 70
-
Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.56 0.83
-
N-acetyl-D-mannosamine the reaction mixture contains 1 mM MgCl2 in 50 mM Tris-acetate buffer, pH 7.5, at 37°C Neisseria meningitidis
2.5.1.57 0.67
-
D-mannose 6-phosphate in 50 mM bicine pH 7.5 at 37°C Homo sapiens
2.5.1.57 1.31
-
N-acetyl-D-mannosamine 6-phosphate in 50 mM bicine pH 7.5 at 37°C Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.56 7.5
-
-
Neisseria meningitidis
2.5.1.57 7.5
-
-
Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.56 7 9
-
Neisseria meningitidis
2.5.1.57 6 10
-
Homo sapiens