EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.56 | expressed in Escherichia coli BL21(DE3) Gold cells | Neisseria meningitidis |
2.5.1.57 | expressed in Escherichia coli BL21 cells | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.5.1.56 | C182A | no activity | Neisseria meningitidis |
2.5.1.56 | C182S | no activity | Neisseria meningitidis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.57 | Ba2+ | 1 mM, 93% decrease of activity | Homo sapiens | |
2.5.1.57 | Ca2+ | 1 mM, 76% decrease of activity | Homo sapiens | |
2.5.1.57 | Co2+ | 1 mM, 15% decrease of activity | Homo sapiens | |
2.5.1.57 | Cu2+ | 1 mM, 68% decrease of activity | Homo sapiens | |
2.5.1.57 | EDTA | 10 mM, complete inhibition | Homo sapiens | |
2.5.1.57 | Fe2+ | 1 mM, 79% decrease of activity | Homo sapiens | |
2.5.1.57 | Ni2+ | 1 mM, 24% decrease of activity | Homo sapiens | |
2.5.1.57 | Zn2+ | 1 mM, 87% decrease of activity | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.56 | 11.6 | - |
N-acetyl-D-mannosamine | the reaction mixture contains 1 mM MgCl2 in 50 mM Tris-acetate buffer, pH 7.5, at 37°C | Neisseria meningitidis | |
2.5.1.57 | 1.04 | - |
N-acetyl-D-mannosamine 6-phosphate | in 50 mM bicine pH 7.5 at 37°C | Homo sapiens | |
2.5.1.57 | 2.62 | - |
D-mannose 6-phosphate | in 50 mM bicine pH 7.5 at 37°C | Homo sapiens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.56 | Co2+ | 1 mM, 271% relative activity compared to Mg2+ | Neisseria meningitidis | |
2.5.1.56 | Mg2+ | 1 mM, required for 100% activity | Neisseria meningitidis | |
2.5.1.56 | Mn2+ | 1 mM, 115% relative activity compared to Mg2+ | Neisseria meningitidis | |
2.5.1.57 | Cd2+ | 1 mM, 30% increase of activity | Homo sapiens | |
2.5.1.57 | Mn2+ | 1 mM, 15% increase of activity | Homo sapiens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.5.1.56 | 38629 | - |
1 * 38629, calculated from amino acid sequence | Neisseria meningitidis |
2.5.1.56 | 38630 | - |
calculated from amino acid sequence | Neisseria meningitidis |
2.5.1.56 | 38649 | - |
1 * 38649, ESI-MS | Neisseria meningitidis |
2.5.1.56 | 38650 | - |
ESI-MS | Neisseria meningitidis |
2.5.1.56 | 39000 | - |
2 * 39000, SDS-PAGE | Neisseria meningitidis |
2.5.1.56 | 78000 | - |
SDS-PAGE | Neisseria meningitidis |
2.5.1.57 | 42360 | - |
calculated from sequence of cDNA | Homo sapiens |
2.5.1.57 | 42360 | - |
ESI mass spectrometry | Homo sapiens |
2.5.1.57 | 100100 | - |
SDS-PAGE | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.56 | Neisseria meningitidis | - |
- |
- |
2.5.1.57 | Homo sapiens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.56 | Ni2+ affinity column chromatography | Neisseria meningitidis |
2.5.1.57 | Ni2+-Sepharose Fast Flow column chromatography | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.56 | N-acetyl-D-mannosamine + phosphoenolpyruvate + H2O | - |
Neisseria meningitidis | N-acetylneuraminate + phosphate | - |
? | |
2.5.1.57 | D-mannose 6-phosphate + ? | - |
Homo sapiens | ? | - |
? | |
2.5.1.57 | N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O | - |
Homo sapiens | N-acetyl-neuraminate 9-phosphate + phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.5.1.56 | dimer | 2 * 39000, SDS-PAGE | Neisseria meningitidis |
2.5.1.56 | monomer | 1 * 38629, calculated from amino acid sequence | Neisseria meningitidis |
2.5.1.56 | monomer | 1 * 38649, ESI-MS | Neisseria meningitidis |
2.5.1.57 | dimer or trimer | the molecular weight is more than the size of a dimer, but smaller than a trimer | Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.56 | NeuNAc synthase | - |
Neisseria meningitidis |
2.5.1.56 | sialic acid synthase | - |
Neisseria meningitidis |
2.5.1.57 | N-acetylneuraminic acid-9-phosphate synthase | - |
Homo sapiens |
2.5.1.57 | NeuNAc-9-P synthase | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.56 | 37 | - |
- |
Neisseria meningitidis |
2.5.1.57 | 37 | - |
- |
Homo sapiens |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.56 | 10 | 70 | - |
Neisseria meningitidis |
2.5.1.57 | 10 | 70 | - |
Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.56 | 0.83 | - |
N-acetyl-D-mannosamine | the reaction mixture contains 1 mM MgCl2 in 50 mM Tris-acetate buffer, pH 7.5, at 37°C | Neisseria meningitidis | |
2.5.1.57 | 0.67 | - |
D-mannose 6-phosphate | in 50 mM bicine pH 7.5 at 37°C | Homo sapiens | |
2.5.1.57 | 1.31 | - |
N-acetyl-D-mannosamine 6-phosphate | in 50 mM bicine pH 7.5 at 37°C | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.56 | 7.5 | - |
- |
Neisseria meningitidis |
2.5.1.57 | 7.5 | - |
- |
Homo sapiens |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.56 | 7 | 9 | - |
Neisseria meningitidis |
2.5.1.57 | 6 | 10 | - |
Homo sapiens |