EC Number | Application | Comment | Organism |
---|---|---|---|
1.10.5.1 | medicine | anti-cancer therapy | Homo sapiens |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.10.5.1 | expressed in Escherichia coli | Homo sapiens |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.10.5.1 | - |
Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.10.5.1 | N161H | results in the total loss of the enzymatic activity towards activation of 5-(aziridin-1-yl)-2,4-dinitrobenzamide, whereas the rates of reduction towards menadione are not altered | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.10.5.1 | 0.061 | - |
5-(aziridin-1-yl)-2,4-dinitrobenzamide | at 25°C in pH 8.5 buffer (50 mM Tris, 140 mM NaCl, and 0.1% Tween 20) with 0.1 mM menadione as co-substrate | Homo sapiens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.10.5.1 | Zn2+ | - |
Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.10.5.1 | Homo sapiens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.10.5.1 | DEAE Sepharose, Superdex 75 column chromatography and Mono-Q column chromatography | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.10.5.1 | 5-(aziridin-1-yl)-2,4-dinitrobenzamide + menadiol | - |
Homo sapiens | ? + reduced menadiol | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.10.5.1 | NQO2 | - |
Homo sapiens |
1.10.5.1 | NRH:quinone oxireductase 2 | - |
Homo sapiens |
1.10.5.1 | QR2 | - |
Homo sapiens |
1.10.5.1 | quinone reductase 2 | - |
Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.10.5.1 | 6 | - |
5-(aziridin-1-yl)-2,4-dinitrobenzamide | at 25°C in pH 8.5 buffer (50 mM Tris, 140 mM NaCl, and 0.1% Tween 20) with 0.1 mM menadione as co-substrate | Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.10.5.1 | FAD | - |
Homo sapiens |