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Literature summary extracted from

  • Fu, Y.; Buryanovskyy, L.; Zhang, Z.
    Crystal structure of quinone reductase 2 in complex with cancer prodrug CB1954 (2005), Biochem. Biophys. Res. Commun., 336, 332-338.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.10.5.1 medicine anti-cancer therapy Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.10.5.1 expressed in Escherichia coli Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.10.5.1
-
Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.10.5.1 N161H results in the total loss of the enzymatic activity towards activation of 5-(aziridin-1-yl)-2,4-dinitrobenzamide, whereas the rates of reduction towards menadione are not altered Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.5.1 0.061
-
5-(aziridin-1-yl)-2,4-dinitrobenzamide at 25°C in pH 8.5 buffer (50 mM Tris, 140 mM NaCl, and 0.1% Tween 20) with 0.1 mM menadione as co-substrate Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.5.1 Zn2+
-
Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.10.5.1 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.5.1 DEAE Sepharose, Superdex 75 column chromatography and Mono-Q column chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.5.1 5-(aziridin-1-yl)-2,4-dinitrobenzamide + menadiol
-
Homo sapiens ? + reduced menadiol
-
?

Synonyms

EC Number Synonyms Comment Organism
1.10.5.1 NQO2
-
Homo sapiens
1.10.5.1 NRH:quinone oxireductase 2
-
Homo sapiens
1.10.5.1 QR2
-
Homo sapiens
1.10.5.1 quinone reductase 2
-
Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.5.1 6
-
5-(aziridin-1-yl)-2,4-dinitrobenzamide at 25°C in pH 8.5 buffer (50 mM Tris, 140 mM NaCl, and 0.1% Tween 20) with 0.1 mM menadione as co-substrate Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.10.5.1 FAD
-
Homo sapiens