Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sawada, Y.; Ayabe, S.
    Multiple mutagenesis of P450 isoflavonoid synthase reveals a key active-site residue (2005), Biochem. Biophys. Res. Commun., 330, 907-913.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.87 expressed in Saccharomyces cerevisiae strain BJ2168 Glycyrrhiza echinata

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.87 L371V inactive Glycyrrhiza echinata
1.14.14.87 L371V/K375T the mutant shows 3-5% activity levels compared to the wild type. The mutant produces a mixture of 95% 3beta-hydroxyflavanone and 5% flavone from (2S)-liquiritigenin Glycyrrhiza echinata
1.14.14.87 S310T/K375T the P450 level of the mutant is approximately 1.5times that of wild type. The mutant shows 3-5% activity levels compared to the wild type Glycyrrhiza echinata
1.14.14.87 S310T/L371V/K375T the mutant produces 100% flavone from (2S)-liquiritigenin Glycyrrhiza echinata
1.14.14.87 S310T/L371V/K375T the mutant shows 4times higher P450 level than the wild type Glycyrrhiza echinata

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.87 Glycyrrhiza echinata
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.87 (2S)-7,4'-dihydroxyflavanone + [reduced NADPH-hemoprotein reductase] + O2 i.e. (2S)-liquiritigenin Glycyrrhiza echinata 2,7,4'-trihydroxyisoflavanone + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.87 2-hydroxyisoflavanone synthase
-
 Glycyrrhiza echinata
1.14.14.87 2HI synthase
-
 Glycyrrhiza echinata
1.14.14.87 CYP93C
-
 Glycyrrhiza echinata
1.14.14.87 IFS
-
 Glycyrrhiza echinata