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Literature summary extracted from

  • Ingram-Smith, C.; Smith, K.S.
    AMP-forming acetyl-CoA synthetases in Archaea show unexpected diversity in substrate utilization (2007), Archaea, 2, 95-107.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.1 expression in Escherichia coli Methanothermobacter thermautotrophicus
6.2.1.1 expression in Escherichia coli, AF-ACS2 Archaeoglobus fulgidus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.1 0.19
-
CoA
-
Methanothermobacter thermautotrophicus
6.2.1.1 0.3
-
CoA AF-ACS2 Archaeoglobus fulgidus
6.2.1.1 1.7
-
acetate AF-ACS2 Archaeoglobus fulgidus
6.2.1.1 2.9
-
ATP AF-ACS2 Archaeoglobus fulgidus
6.2.1.1 3.3
-
ATP
-
Methanothermobacter thermautotrophicus
6.2.1.1 3.5
-
acetate
-
Methanothermobacter thermautotrophicus
6.2.1.1 3.9
-
propionate AF-ACS2 Archaeoglobus fulgidus
6.2.1.1 36.5
-
propionate
-
Methanothermobacter thermautotrophicus
6.2.1.1 133
-
Butyrate AF-ACS2 Archaeoglobus fulgidus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.1 Ca2+ about 30% of the activation with Mg2+ or Mn2+ Methanothermobacter thermautotrophicus
6.2.1.1 Ca2+ about 70% of the activation with Mg2+ or Mn2+, AF-ACS2 Archaeoglobus fulgidus
6.2.1.1 Co2+ about 70% of the activation with Mg2+ or Mn2+, AF-ACS2 Archaeoglobus fulgidus
6.2.1.1 Co2+ about 80% of the activation with Mg2+ or Mn2+ Methanothermobacter thermautotrophicus
6.2.1.1 Mg2+ AF-ACS2 shows strong preference for Mg2+ and Mn2+ as the divalent metal Archaeoglobus fulgidus
6.2.1.1 Mg2+ strong preference for Mg2+ and Mn2+ as the divalent metal Methanothermobacter thermautotrophicus
6.2.1.1 Mn2+ AF-ACS2 shows strong preference for Mg2+ and Mn2+ as the divalent metal Archaeoglobus fulgidus
6.2.1.1 Mn2+ strong preference for Mg2+ and Mn2+ as the divalent metal Methanothermobacter thermautotrophicus
6.2.1.1 Ni2+ about 25% of the activation with Mg2+ or Mn2+ Methanothermobacter thermautotrophicus
6.2.1.1 Ni2+ about 35% of the activation with Mg2+ or Mn2+, AF-ACS2 Archaeoglobus fulgidus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.1 71556
-
2 * 71556, calculated from sequence Methanothermobacter thermautotrophicus
6.2.1.1 77587
-
3 * 77587, AF-ACS2, calculated from sequence Archaeoglobus fulgidus
6.2.1.1 144800
-
gel filtration Methanothermobacter thermautotrophicus
6.2.1.1 221200
-
AF-ACS2, gel filtration Archaeoglobus fulgidus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.1 ATP + acetate + CoA Archaeoglobus fulgidus acetate-CoA ligase is a key enzyme for conversion of acetate to acetyl-CoA AMP + diphosphate + acetyl-CoA
-
?
6.2.1.1 ATP + acetate + CoA Methanothermobacter thermautotrophicus acetate-CoA ligase is a key enzyme for conversion of acetate to acetyl-CoA AMP + diphosphate + acetyl-CoA
-
?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.1 Archaeoglobus fulgidus
-
-
-
6.2.1.1 Methanothermobacter thermautotrophicus Q2XNL6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.1
-
Methanothermobacter thermautotrophicus
6.2.1.1 AF-ACS2 Archaeoglobus fulgidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.1 ATP + acetate + CoA acetate-CoA ligase is a key enzyme for conversion of acetate to acetyl-CoA Archaeoglobus fulgidus AMP + diphosphate + acetyl-CoA
-
?
6.2.1.1 ATP + acetate + CoA acetate-CoA ligase is a key enzyme for conversion of acetate to acetyl-CoA Methanothermobacter thermautotrophicus AMP + diphosphate + acetyl-CoA
-
?
6.2.1.1 ATP + acetate + CoA AF-ACS2 has 2.3fold higher affinity and catalytic efficiency with acetate than with propionate. Enzyme shows a strong preference for ATP versus CTP, GTP, TTP, UTP, ITP or ADP, for which less than 5% activity is observed Archaeoglobus fulgidus AMP + diphosphate + acetyl-CoA
-
?
6.2.1.1 ATP + acetate + CoA MT-ACS1 is limited to acetate and propionate as acyl substrates. MT-ACS1 has nearly 11fold higher affinity and 14fold higher catalytic efficiency with acetate than with propionate. Enzyme shows a strong preference for ATP versus CTP, GTP, TTP, UTP, ITP or ADP, for which less than 5% activity is observed Methanothermobacter thermautotrophicus AMP + diphosphate + acetyl-CoA
-
?
6.2.1.1 ATP + butyrate + CoA AF-ACS2 Archaeoglobus fulgidus AMP + diphosphate + butyryl-CoA
-
?
6.2.1.1 ATP + propionate + CoA AF-ACS2 has 2.3fold higher affinity and catalytic efficiency with acetate than with propionate. Enzyme shows a strong preference for ATP versus CTP, GTP, TTP, UTP, ITP or ADP, for which less than 5% activity is observed Archaeoglobus fulgidus AMP + diphosphate + propionyl-CoA
-
?
6.2.1.1 ATP + propionate + CoA MT-ACS1 is limited to acetate and propionate as acyl substrates. MT-ACS1 has nearly 11fold higher affinity and 14fold higher catalytic efficiency with acetate than with propionate. Enzyme shows a strong preference for ATP versus CTP, GTP, TTP, UTP, ITP or ADP, for which less than 5% activity is observed Methanothermobacter thermautotrophicus AMP + diphosphate + propionyl-CoA
-
?

Subunits

EC Number Subunits Comment Organism
6.2.1.1 dimer 2 * 71556, calculated from sequence Methanothermobacter thermautotrophicus
6.2.1.1 trimer 3 * 77587, AF-ACS2, calculated from sequence Archaeoglobus fulgidus

Synonyms

EC Number Synonyms Comment Organism
6.2.1.1 acetate:CoA ligase (AMP-forming)
-
Archaeoglobus fulgidus
6.2.1.1 acetate:CoA ligase (AMP-forming)
-
Methanothermobacter thermautotrophicus
6.2.1.1 adenosine monophosphate-forming acetyl-CoA synthetase
-
Archaeoglobus fulgidus
6.2.1.1 adenosine monophosphate-forming acetyl-CoA synthetase
-
Methanothermobacter thermautotrophicus
6.2.1.1 AF-ACS2
-
Archaeoglobus fulgidus
6.2.1.1 AMP-forming acetyl-CoA synthetase
-
Archaeoglobus fulgidus
6.2.1.1 AMP-forming acetyl-CoA synthetase
-
Methanothermobacter thermautotrophicus
6.2.1.1 MT-ACS1
-
Methanothermobacter thermautotrophicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.2.1.1 65 70
-
Methanothermobacter thermautotrophicus
6.2.1.1 65 70 AF-ACS2 Archaeoglobus fulgidus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6.2.1.1 45 85 45°C: about 55% of maximal activity, 85°C: about 60% of maximal activity, AF-ACS2 Archaeoglobus fulgidus
6.2.1.1 45 75 45°C: about 55% of maximal activity, 75°C: about 50% of maximal activity Methanothermobacter thermautotrophicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.2.1.1 0.013
-
Butyrate AF-ACS2 Archaeoglobus fulgidus
6.2.1.1 0.7
-
ATP
-
Methanothermobacter thermautotrophicus
6.2.1.1 9.1
-
propionate AF-ACS2 Archaeoglobus fulgidus
6.2.1.1 13.2
-
ATP AF-ACS2 Archaeoglobus fulgidus
6.2.1.1 21.2
-
acetate AF-ACS2 Archaeoglobus fulgidus
6.2.1.1 46.3
-
propionate
-
Methanothermobacter thermautotrophicus
6.2.1.1 65.4
-
acetate
-
Methanothermobacter thermautotrophicus
6.2.1.1 66.6
-
ATP
-
Methanothermobacter thermautotrophicus
6.2.1.1 81.6
-
CoA
-
Methanothermobacter thermautotrophicus
6.2.1.1 144.9
-
CoA AF-ACS2 Archaeoglobus fulgidus