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Literature summary extracted from

  • Yu, F.; Hasebe, F.; Inoue, S.; Mathenge, E.G.; Morita, K.
    Identification and characterization of RNA-dependent RNA polymerase activity in recombinant Japanese encephalitis virus NS5 protein (2007), Arch. Virol., 152, 1859-1869.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.48 expressed in Escherichia coli XL1 Blue cells Japanese encephalitis virus
2.7.7.48 expression of wild-type and mutant enzymes in Escherichia coli Japanese encephalitis virus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.48 D536A inactive Japanese encephalitis virus
2.7.7.48 D536A completely inactive enzyme Japanese encephalitis virus
2.7.7.48 D668A inactive Japanese encephalitis virus
2.7.7.48 D668A completely inactive enzyme Japanese encephalitis virus
2.7.7.48 D669A inactive Japanese encephalitis virus
2.7.7.48 D669A completely inactive enzyme Japanese encephalitis virus
2.7.7.48 D669N reduction in activity to about 5% relative to the parental NS5 Japanese encephalitis virus
2.7.7.48 D669N mutant shows a reduction in activity to about 5% relative to the wild type enzyme Japanese encephalitis virus
2.7.7.48 G605A inactive Japanese encephalitis virus
2.7.7.48 G605A completely inactive enzyme Japanese encephalitis virus
2.7.7.48 K691A inactive Japanese encephalitis virus
2.7.7.48 K691A completely inactive enzyme Japanese encephalitis virus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.48 Ca2+ no activity at 3 mM Japanese encephalitis virus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.48 Mg2+ dependent on divalent cations, Mn2+ is 20times more effective than Mg2+ at optimal concentration (3 mM) Japanese encephalitis virus
2.7.7.48 Mg2+ Mg2+ is 20 times less effective than Mn2+ in coordinating the catalytic reaction of RdRp Japanese encephalitis virus
2.7.7.48 Mn2+ dependent on divalent cations, Mn2+ is 20times more effective than Mg2+ at optimal concentration (3 mM) Japanese encephalitis virus
2.7.7.48 Mn2+ Mn2+ is 20 times more effective than Mg2+ in coordinating the catalytic reaction of RdRp Japanese encephalitis virus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.48 103000
-
 SDS-PAGE Japanese encephalitis virus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.48 nucleoside triphosphate + RNAn Japanese encephalitis virus JEV NS5 protein can initiate RNA synthesis through a de novo initiation mechanism. JEV NS5 protein is more efficient in using negative-strand RNA templates, indicating that the JEV NS5 protein is involved in regulating the ratio of positive strand RNA to negative strand RNA diphosphate + RNAn+1
-
 ?
2.7.7.48 nucleoside triphosphate + RNAn Japanese encephalitis virus JaOH0566 JEV NS5 protein can initiate RNA synthesis through a de novo initiation mechanism. JEV NS5 protein is more efficient in using negative-strand RNA templates, indicating that the JEV NS5 protein is involved in regulating the ratio of positive strand RNA to negative strand RNA diphosphate + RNAn+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.48 Japanese encephalitis virus
-
-
-
2.7.7.48 Japanese encephalitis virus JaOH0566
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.48
-
 Japanese encephalitis virus
2.7.7.48 Talon IMAC resin column chromatography Japanese encephalitis virus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.48 nucleoside triphosphate + RNAn JEV NS5 protein can initiate RNA synthesis through a de novo initiation mechanism. JEV NS5 protein is more efficient in using negative-strand RNA templates, indicating that the JEV NS5 protein is involved in regulating the ratio of positive strand RNA to negative strand RNA Japanese encephalitis virus diphosphate + RNAn+1
-
 ?
2.7.7.48 nucleoside triphosphate + RNAn JEV NS5 protein can initiate RNA synthesis through a de novo initiation mechanism. JEV NS5 protein is more efficient in using negative-strand RNA templates Japanese encephalitis virus diphosphate + RNAn+1
-
 ?
2.7.7.48 nucleoside triphosphate + RNAn uses JEV and dengue-2 virus 3' end plus- and minus-strand RNA templates, the incorporation of [32P]-UMP is much lower when using positive-strand RNA as template than when using negative-strand RNA - an almost 10fold difference in efficiency Japanese encephalitis virus diphosphate + RNAn+1
-
 ?
2.7.7.48 nucleoside triphosphate + RNAn JEV NS5 protein can initiate RNA synthesis through a de novo initiation mechanism. JEV NS5 protein is more efficient in using negative-strand RNA templates, indicating that the JEV NS5 protein is involved in regulating the ratio of positive strand RNA to negative strand RNA Japanese encephalitis virus JaOH0566 diphosphate + RNAn+1
-
 ?
2.7.7.48 nucleoside triphosphate + RNAn JEV NS5 protein can initiate RNA synthesis through a de novo initiation mechanism. JEV NS5 protein is more efficient in using negative-strand RNA templates Japanese encephalitis virus JaOH0566 diphosphate + RNAn+1
-
 ?
2.7.7.48 nucleoside triphosphate + RNAn uses JEV and dengue-2 virus 3' end plus- and minus-strand RNA templates, the incorporation of [32P]-UMP is much lower when using positive-strand RNA as template than when using negative-strand RNA - an almost 10fold difference in efficiency Japanese encephalitis virus JaOH0566 diphosphate + RNAn+1
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.48 ? x * 103000, SDS-PAGE Japanese encephalitis virus

Synonyms

EC Number Synonyms Comment Organism
2.7.7.48 JEV NS5 protein
-
 Japanese encephalitis virus
2.7.7.48 NS5 protein
-
 Japanese encephalitis virus
2.7.7.48 RDRP
-
 Japanese encephalitis virus
2.7.7.48 RNA-dependent RNA polymerase
-
 Japanese encephalitis virus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.48 30
-
-
 Japanese encephalitis virus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.7.48 25 40 25°C: about 55% of maximal activity, 40°C: about 20% of maximal activity Japanese encephalitis virus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.48 7.5 8
-
 Japanese encephalitis virus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.48 6 9 pH 6: about 10% of maximal activity, pH 9: about 20% of maximal activity Japanese encephalitis virus