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Literature summary extracted from

  • Velasco-Garcia, R.; Villalobos, M.A.; Ramirez-Romero, M.A.; Mujica-Jimenez, C.; Iturriaga, G.; Munoz-Clares, R.A.
    Betaine aldehyde dehydrogenase from Pseudomonas aeruginosa: cloning, over-expression in Escherichia coli, and regulation by choline and salt (2006), Arch. Microbiol., 185, 14-22.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.2.1.8 medicine Pseudomonas aeruginosa is an important pathogen, glycine betaine could play an important physiological role under the conditions present in the infection sites Pseudomonas aeruginosa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.8 the gene betB, encoding for Pseudomonas aeruginosa betaine-aldehyde dehydrogenase, is cloned into a pCAL-n vector for sequencing and protein expression in Escherichia coli Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.8 53330
-
 monomer, amino acid sequence Pseudomonas aeruginosa
1.2.1.8 61000
-
 SDS-PAGE, Coomassie blue staining and Western blot Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.8 betaine aldehyde + NAD+ + H2O Pseudomonas aeruginosa
-
 glycine betaine + NADH + 2 H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.8 Pseudomonas aeruginosa Q9HTJ1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.8 purified on Q-Sepharose fast flow and 2', 5'-ADP-Sepharose columns, 28 mg of pure protein per liter of culture are yielded Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.8 0.006
-
 culture conditions, 11.1 mM glucose and 15 mM NH4Cl Pseudomonas aeruginosa
1.2.1.8 0.012
-
 culture conditions, 11.1 mM glucose, 15 mM NH4Cl and 0.4 M NaCl Pseudomonas aeruginosa
1.2.1.8 0.211
-
 culture conditions, 11.1 mM glucose, 20 mM choline and 0.4 M NaCl Pseudomonas aeruginosa
1.2.1.8 0.215
-
 culture conditions, 11.1 mM glucose and 20 mM choline Pseudomonas aeruginosa
1.2.1.8 0.492
-
 culture conditions, 20 mM choline and 0.4 M NaCl Pseudomonas aeruginosa
1.2.1.8 0.571
-
 culture conditions, 20 mM choline Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.8 betaine aldehyde + NAD+ + H2O
-
 Pseudomonas aeruginosa glycine betaine + NADH + 2 H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.2.1.8 tetramer 4 x 61000, SDS-PAGE Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
1.2.1.8 BADH
-
 Pseudomonas aeruginosa
1.2.1.8 betaine aldehyde:NAD(P)+ oxidoreductase
-
 Pseudomonas aeruginosa
1.2.1.8 betaine-aldehyde dehydrogenase
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.8 30
-
 activity assay Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.8 8
-
 activity assay Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.8 NAD+
-
 Pseudomonas aeruginosa