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Literature summary extracted from

  • Sharma, L.K.; Madina, B.R.; Chaturvedi, P.; Sangwan, R.S.; Tuli, R.
    Molecular cloning and characterization of one member of 3beta-hydroxy sterol glucosyltransferase gene family in Withania somnifera (2007), Arch. Biochem. Biophys., 460, 48-55.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.173 expressed in Escherichia coli strain DH5alpha Arabidopsis thaliana
2.4.1.173 the open reading frame of SGTL1 is expressed in Escherichia coli strain DH5alpha Withania somnifera

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.173 0.003
-
 solasodine
-
 Arabidopsis thaliana
2.4.1.173 0.004
-
 ergosterol activity is assayed radiometrically using UDP-[U-14C] glucose, at pH 8.0 and 37°C Withania somnifera
2.4.1.173 0.0045
-
 brassicasterol activity is assayed radiometrically using UDP-[U-14C] glucose, at pH 8.0 and 37°C Withania somnifera
2.4.1.173 0.006
-
 pregnenolone activity is assayed radiometrically using UDP-[U-14C] glucose, at pH 8.0 and 37°C Withania somnifera
2.4.1.173 0.007
-
 3beta-hydroxy-pregna-5,16-dien-20-one
-
 Arabidopsis thaliana
2.4.1.173 0.007
-
 solasodine activity is assayed radiometrically using UDP-[U-14C] glucose, at pH 8.0 and 37°C Withania somnifera
2.4.1.173 0.008
-
 pregnenolone
-
 Arabidopsis thaliana
2.4.1.173 0.008
-
 UDP-glucose activity is assayed radiometrically using UDP-[U-14C] glucose, at pH 8.0 and 37°C Withania somnifera
2.4.1.173 0.01
-
 3beta-hydroxy-16,17alpha-epoxypregnenolone activity is assayed radiometrically using UDP-[U-14C] glucose, at pH 8.0 and 37°C Withania somnifera
2.4.1.173 0.01
-
 3beta-hydroxy-pregna-5,16-dien-one activity is assayed radiometrically using UDP-[U-14C] glucose, at pH 8.0 and 37°C Withania somnifera
2.4.1.173 0.017
-
 3beta-hydroxy-16,17-alpha-epoxypregnenolene
-
 Arabidopsis thaliana
2.4.1.173 0.025
-
 UDP-glucose
-
 Arabidopsis thaliana
2.4.1.173 0.025
-
 transandrosterone activity is assayed radiometrically using UDP-[U-14C] glucose, at pH 8.0 and 37°C Withania somnifera
2.4.1.173 0.027
-
 stigmasterol activity is assayed radiometrically using UDP-[U-14C] glucose, at pH 8.0 and 37°C Withania somnifera
2.4.1.173 0.03
-
 brassicasterol
-
 Arabidopsis thaliana
2.4.1.173 0.035
-
 ergosterol
-
 Arabidopsis thaliana
2.4.1.173 0.04
-
 dehydroepiandrosterone activity is assayed radiometrically using UDP-[U-14C] glucose, at pH 8.0 and 37°C Withania somnifera
2.4.1.173 0.04
-
 beta-sitosterol activity is assayed radiometrically using UDP-[U-14C] glucose, at pH 8.0 and 37°C Withania somnifera
2.4.1.173 0.043
-
 dehydroepiandrosterone
-
 Arabidopsis thaliana
2.4.1.173 0.043
-
 stigmasterol
-
 Arabidopsis thaliana
2.4.1.173 0.056
-
 cholesterol
-
 Arabidopsis thaliana
2.4.1.173 0.059
-
 beta-sitosterol
-
 Arabidopsis thaliana
2.4.1.173 0.059
-
 transandrosterone
-
 Arabidopsis thaliana
2.4.1.173 0.063
-
 5alpha-Cholestan-3beta-ol
-
 Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.173 78000
-
 SDS-PAGE Withania somnifera

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.173 Arabidopsis thaliana
-
-
-
2.4.1.173 Withania somnifera Q2I015 the incubation of leaf discs in the presence of salicylic acid results in transient accumulation of SGTL1 transcript. It increases gradually to reach the maxima of 2.15fold at 12 h followed by a decline.
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.173 gel filtration and ion exchange column chromatography Arabidopsis thaliana
2.4.1.173 partially purified by gel filtration chromatography, resutlts in 3.73fold purification Withania somnifera

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.173 leaf from 4 weeks old plants, young and mature, 2.21fold higher enzyme level than in the stem, SGTL1 transcript accumulates at a higher level in mature leaves (2.41fold) than in the young leaves and seedlings Withania somnifera
-
2.4.1.173 root from 4 weeks old plants, 3.13fold higher expression of the enzyme than in the stem Withania somnifera
-
2.4.1.173 seedling lower expression of the enzyme than in mature leaves Withania somnifera
-
2.4.1.173 stem from 4 weeks old plants, expressend in significantly lower level than in leaves and roots Withania somnifera
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.173 additional information
-
 the activity obtained with dehydroepiandrosterone is 100%. 3-beta-hydroxy-pregna-5,16-dien-one, transandrosterone, 3-beta-hydroxy-16, 17-alpha-epoxy-pregnenolone, pregnenolone, stigmasterol, beta-sitosterol, ergosterol, brassicasterol and solasodine give activities with the enzyme SGTL1. 5-alpha-cholestan-3-beta-ol and cholesterol show no activity with the enzyme. Activity is assayed radiometrically using UDP-[U-14C] glucose, at pH 8.0 and 37°C Withania somnifera

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.173 3beta-hydroxy-16,17alpha-epoxypregnenolone + UDP-alpha-D-glucose
-
 Arabidopsis thaliana 3beta-hydroxy-16,17alpha-epoxypregnenolone 3-beta-D-glucoside + UDP
-
 ?
2.4.1.173 3beta-hydroxy-pregna-5,16-dien-20-one + UDP-glucose
-
 Arabidopsis thaliana UDP + 3beta-20-oxopregna-5,16-dien-3-yl beta-D-glucopyranoside
-
 ?
2.4.1.173 5alpha-cholestan-3beta-ol + UDP-glucose
-
 Arabidopsis thaliana UDP + 3beta,5alpha-cholestan 3-beta-D-glucoside
-
 ?
2.4.1.173 beta-sitosterol + UDP-glucose
-
 Arabidopsis thaliana UDP + beta-sitosterol 3-beta-D-glucoside
-
 ?
2.4.1.173 brassicasterol + UDP-glucose
-
 Arabidopsis thaliana UDP + brassicasterol 3-beta-D-glucoside
-
 ?
2.4.1.173 cholesterol + UDP-glucose
-
 Arabidopsis thaliana UDP + cholesterol 3-beta-D-glucoside
-
 ?
2.4.1.173 dehydroepiandrosterone + UDP-glucose
-
 Arabidopsis thaliana UDP + dehydroepiandrosterone 3-beta-D-glucoside
-
 ?
2.4.1.173 ergosterol + UDP-glucose
-
 Arabidopsis thaliana UDP + ergosterol 3-beta-D-glucoside
-
 ?
2.4.1.173 additional information the enzyme only shows activity with sterols having hydroxyl group at C-3 position. SGTL1 is more active toward sterols without side chain. Utilises only UDP-glucose, UDP-galactose can not serve as the sugar donor. Withania somnifera ?
-
 ?
2.4.1.173 additional information the enzyme only shows activity with sterols having hydroxyl group at C-3 position. Utilises only UDP-glucose, UDP-galactose cannot serve as the sugar donor. Arabidopsis thaliana ?
-
 ?
2.4.1.173 pregnenolene + UDP-glucose
-
 Arabidopsis thaliana UDP + pregnenolone 3-beta-D-glucoside
-
 ?
2.4.1.173 solasodine + UDP-glucose
-
 Arabidopsis thaliana UDP + solasodine 3-beta-D-glucoside
-
 ?
2.4.1.173 stigmasterol + UDP-glucose
-
 Arabidopsis thaliana UDP + stigmasterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + 3beta-hydroxy-16,17alpha-epoxypregnenolone
-
 Withania somnifera ?
-
 ?
2.4.1.173 UDP-glucose + 3beta-hydroxy-16,17alpha-epoxypregnenolone
-
 Arabidopsis thaliana UDP + 3beta-hydroxy-16,17alpha-epoxypregnenolone 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + 3beta-hydroxy-pregna-5,16-dien-one
-
 Withania somnifera UDP + 3beta-20-oxopregna-5,16-dien-3-yl beta-D-glucopyranoside
-
 ?
2.4.1.173 UDP-glucose + beta-sitosterol
-
 Withania somnifera UDP + beta-sitosterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + brassicasterol
-
 Withania somnifera UDP + brassicasterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + dehydroepiandrosterone
-
 Withania somnifera UDP + dehydroepiandrosterone 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + ergosterol
-
 Withania somnifera UDP + ergosterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + pregnenolone
-
 Arabidopsis thaliana UDP + pregnenolone 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + pregnenolone
-
 Withania somnifera UDP + pregnenolone 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + solasodine
-
 Withania somnifera UDP + solasodine 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + stigmasterol
-
 Withania somnifera UDP + stigmasterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + transandrosterone
-
 Arabidopsis thaliana UDP + transandrosterone 3beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + transandrosterone
-
 Withania somnifera UDP + transandrosterone 3beta-D-glucoside
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.173 AtSGT sterol glucosyltransferase in Arabidopsos thaliana Arabidopsis thaliana
2.4.1.173 SGT sterol glucosyltransferase Withania somnifera
2.4.1.173 SGTL1
-
 Withania somnifera
2.4.1.173 SGTL1 gene encoding a member of the sterol glucosyltransferase in Withania somnifera Withania somnifera

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.173 additional information
-
 a decrease in the transcript level is observed within 15 min when the plants are shifted from 25 to 42°C Withania somnifera