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Literature summary extracted from

  • Eyzaguirre, J.; Valdebenito, D.; Cardemil, E.
    Pig liver phosphomevalonate kinase: kinetic mechanism (2006), Arch. Biochem. Biophys., 454, 188-195.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.2 additional information
-
 additional information detailed kinetics, Bi Bi sequential ordered mechanism with phosphomevalonate as the first substrate and ADP the last product, the enzyme follows hyperbolic kinetics and a sequential mechanism, initial velocity and isotope exchange at equilibrium measurements, overview Sus scrofa
2.7.4.2 0.012
-
 (R)-5-phosphomevalonate pH 7.5, 30°C Sus scrofa
2.7.4.2 0.043
-
 ATP pH 7.5, 30°C Sus scrofa
2.7.4.2 0.043
-
 ATP ATP in form of MgATP2- Sus scrofa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.2 Mg2+ the enzyme requires cations with Mg2+ being the most effective Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.2 ATP + (R)-5-phosphomevalonate Sus scrofa one of the initial steps in the biosynthesis of steroids and isoprenoids ADP + (R)-5-diphosphomevalonate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.2 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.2 native enzyme from liver Sus scrofa

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.4.2 ATP + (R)-5-phosphomevalonate = ADP + (R)-5-diphosphomevalonate kinetic mechanism, the enzyme follows hyperbolic kinetics and a sequential mechanism Sus scrofa
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.4.2 liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.2 0.16
-
 purified enzyme Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.2 ATP + (R)-5-phosphomevalonate
-
 Sus scrofa ADP + (R)-5-diphosphomevalonate
-
 r
2.7.4.2 ATP + (R)-5-phosphomevalonate one of the initial steps in the biosynthesis of steroids and isoprenoids Sus scrofa ADP + (R)-5-diphosphomevalonate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.4.2 ? x * 21000-22500, SDS-PAGE Sus scrofa

Synonyms

EC Number Synonyms Comment Organism
2.7.4.2 phosphomevalonate kinase
-
 Sus scrofa
2.7.4.2 PMK
-
 Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.4.2 30
-
 assay at Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.2 7.5 9.5
-
 Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.4.2 ADP
-
 Sus scrofa
2.7.4.2 ATP
-
 Sus scrofa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.4.2 additional information
-
 additional information inhibition kinetics, product inhibition patterns Sus scrofa