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Literature summary extracted from

  • de Carvalho, L.P.; Blanchard, J.S.
    Kinetic analysis of the effects of monovalent cations and divalent metals on the activity of Mycobacterium tuberculosis alpha-isopropylmalate synthase (2006), Arch. Biochem. Biophys., 451, 141-148.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.13 Ca2+ 50% inhibition at 0.205 mM Mycobacterium tuberculosis
2.3.3.13 Zn2+ 50% inhibition at 0.031 mM Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.13 0.0017
-
 2-oxo-3-methylbutanoate pH 7.4, 25°C, presence of Mn2+ Mycobacterium tuberculosis
2.3.3.13 0.0022
-
 2-oxo-3-methylbutanoate pH 7.4, 25°C, presence of Co2+ Mycobacterium tuberculosis
2.3.3.13 0.012
-
 2-oxo-3-methylbutanoate pH 7.4, 25°C, presence of Mg2+ Mycobacterium tuberculosis
2.3.3.13 0.016
-
 2-oxo-3-methylbutanoate pH 7.4, 25°C, presence of K+ Mycobacterium tuberculosis
2.3.3.13 0.019
-
 2-oxo-3-methylbutanoate pH 7.4, 25°C, presence of Rb+ Mycobacterium tuberculosis
2.3.3.13 0.037
-
 acetyl-CoA pH 7.4, 25°C, presence of Co2+ Mycobacterium tuberculosis
2.3.3.13 0.05
-
 acetyl-CoA pH 7.4, 25°C, presence of Mn2+ Mycobacterium tuberculosis
2.3.3.13 0.103
-
 acetyl-CoA pH 7.4, 25°C, presence of K+ Mycobacterium tuberculosis
2.3.3.13 0.12
-
 acetyl-CoA pH 7.4, 25°C, presence of Rb+ Mycobacterium tuberculosis
2.3.3.13 0.138
-
 acetyl-CoA pH 7.4, 25°C, presence of Mg2+ Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.3.13 Co2+ ratio kcat/Kact 0.052 per s and M. Detailed kinetic analysis Mycobacterium tuberculosis
2.3.3.13 K+ K+ is the likely physiological allosteric activator Mycobacterium tuberculosis
2.3.3.13 Mg2+ and Mn2+, best activators, ratio kcat/Kact 0.014 per s and M. Detailed kinetic analysis Mycobacterium tuberculosis
2.3.3.13 Mn2+ and Mg2+, best activators, ratio kcat/Kact 0.034 per s and M. Detailed kinetic analysis Mycobacterium tuberculosis
2.3.3.13 Rb+ activation Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.13 Mycobacterium tuberculosis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.13 2-oxo-3-methylbutanoate + acetyl-CoA + H2O
-
 Mycobacterium tuberculosis 3-hydroxy-4-methyl-3-carboxypentanoate + CoA
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.3.13 0.77
-
 2-oxo-3-methylbutanoate pH 7.4, 25°C, presence of Co2+ Mycobacterium tuberculosis
2.3.3.13 1.69
-
 2-oxo-3-methylbutanoate pH 7.4, 25°C, presence of Mn2+ Mycobacterium tuberculosis
2.3.3.13 2.9
-
 2-oxo-3-methylbutanoate pH 7.4, 25°C, presence of Rb+ Mycobacterium tuberculosis
2.3.3.13 3.2
-
 2-oxo-3-methylbutanoate pH 7.4, 25°C, presence of K+ Mycobacterium tuberculosis
2.3.3.13 3.5
-
 2-oxo-3-methylbutanoate pH 7.4, 25°C, presence of Mg2+ Mycobacterium tuberculosis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.3.13 0.00037
-
 Zn2+ pH 7.5, 25°C Mycobacterium tuberculosis
2.3.3.13 0.0025
-
 Ca2+ pH 7.5, 25°C Mycobacterium tuberculosis