BRENDA - Enzyme Database show

Identification and characterization of an arachidonate 11R-lipoxygenase

Mortimer, M.; Jaerving, R.; Brash, AA.R.; Samel, N.; Jaerving, I.; Arch. Biochem. Biophys. 445, 147-155 (2006)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.13.11.31
expressed in Escherichia coli BL21(DE3) cells
Gersemia fruticosa
General Stability
EC Number
General Stability
Organism
1.13.11.31
the enzyme shows higher activity in 50 mM CAPS buffer (pH 10-11) and glycine buffer (pH 8.5-10.5) than in 50 mM Tris-HCl (pH 7-9)
Gersemia fruticosa
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.13.11.31
membrane
-
Gersemia fruticosa
16020
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.13.11.31
Ca2+
10 mM, required for activity
Gersemia fruticosa
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.13.11.31
77000
-
SDS-PAGE
Gersemia fruticosa
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.13.11.31
Gersemia fruticosa
-
soft coral
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.13.11.31
11,14,17-eicosatrienoic acid + O2
poor substrate
671591
Gersemia fruticosa
?
-
-
-
?
1.13.11.31
adrenic acid + O2
-
671591
Gersemia fruticosa
?
-
-
-
?
1.13.11.31
arachidonate + O2
-
671591
Gersemia fruticosa
(5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate
-
-
-
?
1.13.11.31
dihomo-gamma linolenic acid + O2
-
671591
Gersemia fruticosa
?
-
-
-
?
1.13.11.31
docosahexaenoic acid + O2
-
671591
Gersemia fruticosa
14-hydroxydocosahexaenoic acid
-
-
-
?
1.13.11.31
eicosapentaenoic acid + O2
-
671591
Gersemia fruticosa
12-hydroxyeicosapentaenoic acid
-
-
-
?
1.13.11.31
gamma linolenic acid + O2
poor substrate
671591
Gersemia fruticosa
10-hydroxy-octadeca-(6Z,8E,12Z)-trienoic acid
-
-
-
?
1.13.11.31
linolenic acid + O2
poor substrate
671591
Gersemia fruticosa
?
-
-
-
?
1.13.11.31
additional information
no reaction with mead acid and methyl esters of linolenic acid and arachidonate
671591
Gersemia fruticosa
?
-
-
-
-
Temperature Range [C]
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
1.13.11.31
5
15
-
Gersemia fruticosa
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.13.11.31
25
-
significant activity decrease above 25C
Gersemia fruticosa
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.13.11.31
7.5
11
-
Gersemia fruticosa
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.13.11.31
expressed in Escherichia coli BL21(DE3) cells
Gersemia fruticosa
General Stability (protein specific)
EC Number
General Stability
Organism
1.13.11.31
the enzyme shows higher activity in 50 mM CAPS buffer (pH 10-11) and glycine buffer (pH 8.5-10.5) than in 50 mM Tris-HCl (pH 7-9)
Gersemia fruticosa
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.13.11.31
membrane
-
Gersemia fruticosa
16020
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.13.11.31
Ca2+
10 mM, required for activity
Gersemia fruticosa
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.13.11.31
77000
-
SDS-PAGE
Gersemia fruticosa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.13.11.31
11,14,17-eicosatrienoic acid + O2
poor substrate
671591
Gersemia fruticosa
?
-
-
-
?
1.13.11.31
adrenic acid + O2
-
671591
Gersemia fruticosa
?
-
-
-
?
1.13.11.31
arachidonate + O2
-
671591
Gersemia fruticosa
(5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate
-
-
-
?
1.13.11.31
dihomo-gamma linolenic acid + O2
-
671591
Gersemia fruticosa
?
-
-
-
?
1.13.11.31
docosahexaenoic acid + O2
-
671591
Gersemia fruticosa
14-hydroxydocosahexaenoic acid
-
-
-
?
1.13.11.31
eicosapentaenoic acid + O2
-
671591
Gersemia fruticosa
12-hydroxyeicosapentaenoic acid
-
-
-
?
1.13.11.31
gamma linolenic acid + O2
poor substrate
671591
Gersemia fruticosa
10-hydroxy-octadeca-(6Z,8E,12Z)-trienoic acid
-
-
-
?
1.13.11.31
linolenic acid + O2
poor substrate
671591
Gersemia fruticosa
?
-
-
-
?
1.13.11.31
additional information
no reaction with mead acid and methyl esters of linolenic acid and arachidonate
671591
Gersemia fruticosa
?
-
-
-
-
Temperature Range [C] (protein specific)
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
1.13.11.31
5
15
-
Gersemia fruticosa
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.13.11.31
25
-
significant activity decrease above 25C
Gersemia fruticosa
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.13.11.31
7.5
11
-
Gersemia fruticosa