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Literature summary extracted from

  • Fernandes, R.P.; Phaosiri, C.; Proteau, P.J.
    Mutation in the flexible loop of 1-deoxy-D-xylulose 5-phosphate reductoisomerase broadens substrate utilization (2005), Arch. Biochem. Biophys., 444, 159-164.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.267 overexpression of N-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli Synechocystis sp.

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.267 W204A site-directed mutagenesis, the mutant shows reduced activity and altered kinetics compared to the wild-type enzyme Synechocystis sp.
1.1.1.267 W204F site-directed mutagenesis, the mutant shows reduced activity and altered kinetics compared to the wild-type enzyme, the mutant is active with the substrate analogue 1,2-dideoxy-D-threo-3-hexulose 6-phosphate in contrast to the wild-type enzyme Synechocystis sp.
1.1.1.267 W204L site-directed mutagenesis, the mutant shows reduced activity and altered kinetics compared to the wild-type enzyme Synechocystis sp.
1.1.1.267 W204V site-directed mutagenesis, the mutant shows reduced activity and altered kinetics compared to the wild-type enzyme Synechocystis sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.267 1,2-dideoxy-D-threo-3-hexulose 6-phosphate weak, competitive inhibition, a highly conserved tryptophan residue in the flexible loop is blocked by the substrate analog Synechocystis sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.267 0.17
-
 1-deoxy-D-xylulose 5-phosphate pH 7.8, 37°C, recombinant wild-type enzyme Synechocystis sp.
1.1.1.267 0.42
-
 1-deoxy-D-xylulose 5-phosphate pH 7.8, 37°C, recombinant mutant W204F Synechocystis sp.
1.1.1.267 1.3
-
 1,2-dideoxy-D-threo-3-hexulose 6-phosphate pH 7.8, 37°C, recombinant mutant W204F Synechocystis sp.
1.1.1.267 2.9
-
 1-deoxy-D-xylulose 5-phosphate pH 7.8, 37°C, recombinant mutant W204L Synechocystis sp.
1.1.1.267 5
-
 1-deoxy-D-xylulose 5-phosphate pH 7.8, 37°C, recombinant mutant W204V Synechocystis sp.
1.1.1.267 12
-
 1-deoxy-D-xylulose 5-phosphate pH 7.8, 37°C, recombinant mutant W204A Synechocystis sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.267 Mn2+ required Synechocystis sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ Synechocystis sp.
-
 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.267 Synechocystis sp.
-
 strain PCC6803
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.267 recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli by metal ion affinity chromatography Synechocystis sp.

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.267 2-C-methyl-D-erythritol 4-phosphate + NADP+ = 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ the conserved active site residue Trp204 is important in catalysis Synechocystis sp.
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.267 additional information
-
-
 Synechocystis sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.267 1,2-dideoxy-D-threo-3-hexulose 6-phosphate + NADPH + H+ i.e. 1-methyl-1-deoxy-D-xylulose 5-phosphate or M-DXP, a substrate analogue whichis inactive with the wild-type but active with mutant W204F Synechocystis sp. 2-C-ethyl-D-erythritol 4-phosphate + NADP+
-
 ?
1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+
-
 Synechocystis sp. 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 ?
1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ i.e. DXP Synechocystis sp. 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 ?
1.1.1.267 additional information substrate synthesis and product identification method, overview Synechocystis sp. ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.267 DXP reductoisomerase
-
 Synechocystis sp.
1.1.1.267 DXR
-
 Synechocystis sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.267 37
-
 assay at Synechocystis sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.267 0.04
-
 1,2-dideoxy-D-threo-3-hexulose 6-phosphate pH 7.8, 37°C, recombinant mutant W204F Synechocystis sp.
1.1.1.267 0.16
-
 1-deoxy-D-xylulose 5-phosphate pH 7.8, 37°C, recombinant mutant W204A Synechocystis sp.
1.1.1.267 0.26
-
 1-deoxy-D-xylulose 5-phosphate pH 7.8, 37°C, recombinant mutant W204L Synechocystis sp.
1.1.1.267 0.54
-
 1-deoxy-D-xylulose 5-phosphate pH 7.8, 37°C, recombinant mutant W204V Synechocystis sp.
1.1.1.267 10
-
 1-deoxy-D-xylulose 5-phosphate pH 7.8, 37°C, recombinant mutant W204F Synechocystis sp.
1.1.1.267 17
-
 1-deoxy-D-xylulose 5-phosphate pH 7.8, 37°C, recombinant wild-type enzyme Synechocystis sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.267 7.8
-
 assay at Synechocystis sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.267 NADPH
-
 Synechocystis sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.267 0.63
-
 1,2-dideoxy-D-threo-3-hexulose 6-phosphate pH 7.8, 37°C, recombinant wild-type enzyme Synechocystis sp.