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Literature summary extracted from

  • Suliman, H.S.; Sawyer, G.M.; Appling, D.R.; Robertus, J.D.
    Purification and properties of cobalamin-independent methionine synthase from Candida albicans and Saccharomyces cerevisiae (2005), Arch. Biochem. Biophys., 441, 56-63.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.14 expressed in Saccharomyces cerevisiae strain DAY4.1 Saccharomyces cerevisiae
2.1.1.14 expressed in Saccharomyces cerevisiae strain DAY4.1 Candida albicans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.14 0.013
-
 L-homocysteine in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C Saccharomyces cerevisiae
2.1.1.14 0.014
-
 L-homocysteine in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C Candida albicans
2.1.1.14 0.084
-
 5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C Saccharomyces cerevisiae
2.1.1.14 0.094
-
 5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamyl-gamma-glutamylglutamate in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C Saccharomyces cerevisiae
2.1.1.14 0.109
-
 5-methyltetrahydropteroyl-gamma-glutamylglutamate in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C Saccharomyces cerevisiae
2.1.1.14 0.113
-
 5-methyltetrahydropteroyl-gamma-glutamylglutamate in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C Candida albicans
2.1.1.14 0.12
-
 5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamyl-gamma-glutamylglutamate in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C Candida albicans
2.1.1.14 0.129
-
 5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C Candida albicans

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.14 Candida albicans
-
-
-
2.1.1.14 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.14 DEAE Sepharose column chromatography Candida albicans
2.1.1.14 HiTrap heparin column chromatography Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.14 additional information
-
 29.3 units/mg specific activity for 5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate after 5.6fold purification, in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C Saccharomyces cerevisiae
2.1.1.14 additional information
-
 42.11 units/mg specific activity for 5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate after 11.7fold purification, in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C Candida albicans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.14 5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
 Saccharomyces cerevisiae tetrahydropteroyl-gamma-glutamyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
 ?
2.1.1.14 5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
 Candida albicans tetrahydropteroyl-gamma-glutamyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
 ?
2.1.1.14 5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
 Saccharomyces cerevisiae tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
 ?
2.1.1.14 5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
 Saccharomyces cerevisiae tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
 r
2.1.1.14 5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
 Candida albicans tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
 ?
2.1.1.14 5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
 Candida albicans tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
 r
2.1.1.14 5-methyltetrahydropteroyl-gamma-glutamylglutamate + L-homocysteine
-
 Saccharomyces cerevisiae tetrahydropteroyl-gamma-glutamylglutamate + L-methionine
-
 ?
2.1.1.14 5-methyltetrahydropteroyl-gamma-glutamylglutamate + L-homocysteine
-
 Candida albicans tetrahydropteroyl-gamma-glutamylglutamate + L-methionine
-
 ?
2.1.1.14 5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine
-
 Saccharomyces cerevisiae tetrahydropteroyltri-L-glutamate + L-methionine
-
 ?
2.1.1.14 5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine
-
 Candida albicans tetrahydropteroyltri-L-glutamate + L-methionine
-
 ?
2.1.1.14 additional information does not use 5-methyltetrahydropteroylglutamate as a substrate Saccharomyces cerevisiae ?
-
 ?
2.1.1.14 additional information does not use 5-methyltetrahydropteroylglutamate as a substrate Candida albicans ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.14 cobalamin-independent methionine synthase
-
 Saccharomyces cerevisiae
2.1.1.14 cobalamin-independent methionine synthase
-
 Candida albicans
2.1.1.14 Met6p
-
 Saccharomyces cerevisiae
2.1.1.14 Met6p
-
 Candida albicans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.14 0.283
-
 5-methyltetrahydropteroyl-gamma-glutamylglutamate in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C Saccharomyces cerevisiae
2.1.1.14 0.33
-
 L-homocysteine in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C Saccharomyces cerevisiae
2.1.1.14 0.35
-
 5-methyltetrahydropteroyl-gamma-glutamylglutamate in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C Candida albicans
2.1.1.14 0.4
-
 5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C Candida albicans
2.1.1.14 0.417
-
 L-homocysteine in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C Candida albicans
2.1.1.14 0.417
-
 5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamyl-gamma-glutamylglutamate in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C Candida albicans
2.1.1.14 0.533
-
 5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C Saccharomyces cerevisiae
2.1.1.14 0.6
-
 5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamyl-gamma-glutamylglutamate in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.14 additional information vitamin-B12 independent enzyme Saccharomyces cerevisiae
2.1.1.14 additional information vitamin-B12 independent enzyme Candida albicans