Literature summary extracted from

Pucciarelli, S.; Spina, M.; Montecchia, F.; Lupidi, G.; Eleuteri, A.M.; Fioretti, E.; Angeletti, M.
Peroxynitrite-mediated oxidation of the C85S/C152E mutant of dihydrofolate reductase from Escherichia coli: functional and structural effects (2005), Arch. Biochem. Biophys., 434, 221-231.

Protein Variants

EC Number	Protein Variants	Comment	Organism
1.5.1.3	C85S/C152E	cysteine-free mutant, analysis of inhibition by p.eroxynitrite. bicarbonate buffer protects mutant from inhibition by peroxynitrite. Decrease in mutant stability upon oxidation	Escherichia coli

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
1.5.1.3	peroxynitrite	0.1 mM, 50% inhibition of cysteine-free mutant C85S/C152E, no inhibition of wild-type	Escherichia coli

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.5.1.3	Escherichia coli	-	-	-

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Release 2023.1 (January 2023)