Any feedback?
Literature summary extracted from
- Purification and characterization of glucosamine-6-phosphate deaminase from dog kidney cortex (1992), Arch. Biochem. Biophys., 297, 213-220.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.99.6 | additional information | no effect by glucose 6-phosphate | Canis lupus familiaris | |
| 3.5.99.6 | N-acetylglucosamine 6-phosphate | the cooperativity of the reaction is completely abolished by saturating concentrations of GlcNAcGP, this allosteric modulator activated the reaction with a typical K-effect, overview | Canis lupus familiaris |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.99.6 | 2-deoxy-2-amino-D-glucitol 6-phosphate | - |
Canis lupus familiaris | |
| 3.5.99.6 | additional information | no effect by glucose 6-phosphate | Canis lupus familiaris |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.99.6 | additional information | - |
additional information | the enzyme displays positive homotropic cooperativity towards D-glucosamine 6-phosphate | Canis lupus familiaris | |
| 3.5.99.6 | 0.25 | - |
D-glucosamine 6-phosphate | hyperbolic kinetic conditions, in presence of 1 mM N-acetylglucosamine 6-phosphate, pH 8.8 | Canis lupus familiaris | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.99.6 | 30400 | - |
6 * 30400, SDS-PAGE | Canis lupus familiaris |
| 3.5.99.6 | 180000 | - |
about, gel filtration | Canis lupus familiaris |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.99.6 | D-glucosamine 6-phosphate + H2O | Canis lupus familiaris | - |
D-fructose 6-phosphate + NH3 | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.99.6 | Canis lupus familiaris | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.99.6 | native enzyme 1390fold from kidney cortex by affinity chromatography on N-epsilon-amino-n-hexanoyl-D-glucosamine-6-phosphate agarose and on phosphocellulose, the latter behaving as an active site affinity resin | Canis lupus familiaris |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.5.99.6 | kidney | cortex | Canis lupus familiaris | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.99.6 | 85 | - |
purified enzyme | Canis lupus familiaris |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.5.99.6 | 4°C, purified enzyme, pH 5.0-9.0, overnight, in presence of different salts, stable | Canis lupus familiaris |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.99.6 | D-glucosamine 6-phosphate + H2O | - |
Canis lupus familiaris | D-fructose 6-phosphate + NH3 | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.99.6 | hexamer | 6 * 30400, SDS-PAGE | Canis lupus familiaris |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.99.6 | More | formerly EC 5.3.1.10 | Canis lupus familiaris |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.99.6 | 42 | - |
D-glucosamine 6-phosphate | per subunit, hyperbolic kinetic conditions, in presence of 1 mM N-acetylglucosamine 6-phosphate, pH 8.8 | Canis lupus familiaris | |
| 3.5.99.6 | 255 | - |
D-glucosamine 6-phosphate | hexameric enzyme, hyperbolic kinetic conditions, in presence of 1 mM N-acetylglucosamine 6-phosphate, pH 8.8 | Canis lupus familiaris | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.99.6 | 8.8 | - |
assay at | Canis lupus familiaris |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.99.6 | 6 | 10 | pH-profile, overview | Canis lupus familiaris |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.99.6 | 5 | 9 | 4°C, purified enzyme, overnight, in presence of different salts, stable | Canis lupus familiaris |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.5.99.6 | Canis lupus familiaris | isoelectric focusing and chromatofocusing | - |
5.7 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
