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Literature summary extracted from

  • Wu, S.J.; Zhang, L.L.; Wang, J.D.; Chen, J.M.
    Bacillus circulans WZ-12 - a newly discovered aerobic dichloromethane-degrading methylotrophic bacterium (2007), Appl. Microbiol. Biotechnol., 76, 1289-1296.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.5.1.3 20000
-
 estimated by SDS-PAGE Niallia circulans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.5.1.3 dichloromethane + H2O Niallia circulans
-
 formaldehyde + chloride
-
 ?
4.5.1.3 dichloromethane + H2O Methylobacterium sp.
-
 formaldehyde + chloride
-
 ?
4.5.1.3 dichloromethane + H2O Hyphomicrobium sp. DM2
-
 formaldehyde + chloride
-
 ?
4.5.1.3 dichloromethane + H2O Methylorubrum extorquens DM4
-
 formaldehyde + chloride
-
 ?
4.5.1.3 dichloromethane + H2O Niallia circulans WZ12
-
 formaldehyde + chloride
-
 ?
4.5.1.3 dichloromethane + H2O Methylobacterium sp. DM11
-
 formaldehyde + chloride
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.5.1.3 Hyphomicrobium sp. DM2
-
-
-
4.5.1.3 Methylobacterium sp.
-
 DM11
-
4.5.1.3 Methylobacterium sp. DM11
-
 DM11
-
4.5.1.3 Methylorubrum extorquens DM4
-
-
-
4.5.1.3 Niallia circulans
-
-
-
4.5.1.3 Niallia circulans WZ12
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.5.1.3 purified to 8.27-fold with a yield of 34.83%, purified by ammonium sulfate fractionation, gel filtration chromatography and anion-exchange chromatography Niallia circulans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.5.1.3 14.36
-
 crude extract Niallia circulans
4.5.1.3 21.38
-
 after purification by (NH4)2SO4 salting out Niallia circulans
4.5.1.3 49.12
-
 after purification by Sephadex G-75 chromatography Niallia circulans
4.5.1.3 118.8
-
 after purification by anion exchange column Niallia circulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.5.1.3 dichloromethane + H2O
-
 Niallia circulans formaldehyde + chloride
-
 ?
4.5.1.3 dichloromethane + H2O
-
 Methylobacterium sp. formaldehyde + chloride
-
 ?
4.5.1.3 dichloromethane + H2O
-
 Hyphomicrobium sp. DM2 formaldehyde + chloride
-
 ?
4.5.1.3 dichloromethane + H2O
-
 Methylorubrum extorquens DM4 formaldehyde + chloride
-
 ?
4.5.1.3 dichloromethane + H2O
-
 Niallia circulans WZ12 formaldehyde + chloride
-
 ?
4.5.1.3 dichloromethane + H2O
-
 Methylobacterium sp. DM11 formaldehyde + chloride
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.5.1.3 dichloromethane dehalogenase
-
 Niallia circulans
4.5.1.3 dichloromethane dehalogenase
-
 Methylobacterium sp.
4.5.1.3 dichloromethane dehalogenase
-
 Hyphomicrobium sp. DM2
4.5.1.3 dichloromethane dehalogenase
-
 Methylorubrum extorquens DM4

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.5.1.3 0.55
-
 Dichloromethane pH 8.0 and 30°C Hyphomicrobium sp. DM2
4.5.1.3 0.87
-
 Dichloromethane pH 8.0 and 30°C Methylorubrum extorquens DM4
4.5.1.3 3.28
-
 Dichloromethane pH 8.0 and 30°C Methylobacterium sp.
4.5.1.3 3.3
-
 Dichloromethane pH 8.0 and 30°C Niallia circulans
4.5.1.3 4.78
-
 Dichloromethane pH 8.0 and 30°C Niallia circulans

Cofactor

EC Number Cofactor Comment Organism Structure
4.5.1.3 glutathione strictly required Niallia circulans
4.5.1.3 glutathione strictly required Methylobacterium sp.
4.5.1.3 glutathione strictly required Hyphomicrobium sp. DM2
4.5.1.3 glutathione strictly required Methylorubrum extorquens DM4