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Literature summary extracted from

  • Chernyshev, A.; Fleischmann, T.; Kohen, A.
    Thymidyl biosynthesis enzymes as antibiotic targets (2007), Appl. Microbiol. Biotechnol., 74, 282-289.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.1.74 medicine human pathogen, target for new antibiotics Geobacillus stearothermophilus
2.1.1.74 medicine human pathogen, target for new antibiotics Mycobacterium leprae
2.1.1.74 medicine human pathogen, target for new antibiotics Mycobacterium tuberculosis
2.1.1.74 medicine human pathogen, target for new antibiotics Clostridium acidi-urici
2.1.1.74 medicine human pathogen, target for new antibiotics Clostridium botulinum
2.1.1.74 medicine human pathogen, target for new antibiotics Rickettsia sp.
2.1.1.74 medicine human pathogen, target for new antibiotics Bacillus anthracis
2.1.1.74 medicine human pathogen, target for new antibiotics Treponema palladium
2.1.1.148 additional information residues His53, Glu190, Arg90, and Arg182 are essential for TS activity Paramecium bursaria Chlorella virus-1

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.148
-
Mycobacterium tuberculosis
2.1.1.148 crystal structure shows certain small structural differences in the active site when compared with either bacterial FDTS Paramecium bursaria Chlorella virus-1
2.1.1.148 crystal structure shows no structural similarity with “classical” TS Thermotoga maritima

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.148 additional information 5-fluorouracil is not a potential inhibitor Paramecium bursaria Chlorella virus-1
2.1.1.148 NADPH inhibits FDTS at high concentrations at all temperatures, NADPH may not be the natural reducing agent of FDTS Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.74 Bacillus anthracis
-
-
-
2.1.1.74 Clostridium acidi-urici
-
-
-
2.1.1.74 Clostridium botulinum
-
-
-
2.1.1.74 Geobacillus stearothermophilus
-
-
-
2.1.1.74 Mycobacterium leprae
-
-
-
2.1.1.74 Mycobacterium tuberculosis
-
-
-
2.1.1.74 Rickettsia sp.
-
-
-
2.1.1.74 Treponema palladium
-
-
-
2.1.1.148 Bacillus anthracis
-
-
-
2.1.1.148 Chlamydia trachomatis
-
-
-
2.1.1.148 Clostridium botulinum
-
-
-
2.1.1.148 Helicobacter pylori
-
-
-
2.1.1.148 Mycobacterium leprae
-
-
-
2.1.1.148 Mycobacterium tuberculosis P9WG57
-
-
2.1.1.148 Mycobacterium tuberculosis H37Rv P9WG57
-
-
2.1.1.148 no activity in Homo sapiens
-
-
-
2.1.1.148 Paramecium bursaria Chlorella virus-1
-
-
-
2.1.1.148 Pyrococcus furiosus
-
-
-
2.1.1.148 Rickettsia sp.
-
-
-
2.1.1.148 Thermotoga maritima
-
-
-
2.1.1.148 Treponema palladium
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.74 5,10-methylenetetrahydrofolate + tRNA UpsiC + FADH2
-
Geobacillus stearothermophilus tetrahydrofolate + tRNA TpsiC + FAD
-
?
2.1.1.74 5,10-methylenetetrahydrofolate + tRNA UpsiC + FADH2
-
Mycobacterium leprae tetrahydrofolate + tRNA TpsiC + FAD
-
?
2.1.1.74 5,10-methylenetetrahydrofolate + tRNA UpsiC + FADH2
-
Mycobacterium tuberculosis tetrahydrofolate + tRNA TpsiC + FAD
-
?
2.1.1.74 5,10-methylenetetrahydrofolate + tRNA UpsiC + FADH2
-
Clostridium acidi-urici tetrahydrofolate + tRNA TpsiC + FAD
-
?
2.1.1.74 5,10-methylenetetrahydrofolate + tRNA UpsiC + FADH2
-
Clostridium botulinum tetrahydrofolate + tRNA TpsiC + FAD
-
?
2.1.1.74 5,10-methylenetetrahydrofolate + tRNA UpsiC + FADH2
-
Rickettsia sp. tetrahydrofolate + tRNA TpsiC + FAD
-
?
2.1.1.74 5,10-methylenetetrahydrofolate + tRNA UpsiC + FADH2
-
Bacillus anthracis tetrahydrofolate + tRNA TpsiC + FAD
-
?
2.1.1.74 5,10-methylenetetrahydrofolate + tRNA UpsiC + FADH2
-
Treponema palladium tetrahydrofolate + tRNA TpsiC + FAD
-
?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2
-
Chlamydia trachomatis dTMP + tetrahydrofolate + FAD
-
?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2
-
Helicobacter pylori dTMP + tetrahydrofolate + FAD
-
?
2.1.1.148 additional information mRNA binding traits Pyrococcus furiosus ?
-
?

Subunits

EC Number Subunits Comment Organism
2.1.1.148 tetramer
-
Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
2.1.1.74 FDRTS
-
Geobacillus stearothermophilus
2.1.1.74 FDRTS
-
Mycobacterium leprae
2.1.1.74 FDRTS
-
Mycobacterium tuberculosis
2.1.1.74 FDRTS
-
Clostridium acidi-urici
2.1.1.74 FDRTS
-
Clostridium botulinum
2.1.1.74 FDRTS
-
Rickettsia sp.
2.1.1.74 FDRTS
-
Bacillus anthracis
2.1.1.74 FDRTS
-
Treponema palladium
2.1.1.74 folate-dependent ribothymidyl synthase
-
Geobacillus stearothermophilus
2.1.1.74 folate-dependent ribothymidyl synthase
-
Mycobacterium leprae
2.1.1.74 folate-dependent ribothymidyl synthase
-
Mycobacterium tuberculosis
2.1.1.74 folate-dependent ribothymidyl synthase
-
Clostridium acidi-urici
2.1.1.74 folate-dependent ribothymidyl synthase
-
Clostridium botulinum
2.1.1.74 folate-dependent ribothymidyl synthase
-
Rickettsia sp.
2.1.1.74 folate-dependent ribothymidyl synthase
-
Bacillus anthracis
2.1.1.74 folate-dependent ribothymidyl synthase
-
Treponema palladium
2.1.1.148 FDTS
-
Chlamydia trachomatis
2.1.1.148 FDTS
-
Pyrococcus furiosus
2.1.1.148 FDTS
-
Mycobacterium leprae
2.1.1.148 FDTS
-
Helicobacter pylori
2.1.1.148 FDTS
-
Thermotoga maritima
2.1.1.148 FDTS
-
Clostridium botulinum
2.1.1.148 FDTS
-
Rickettsia sp.
2.1.1.148 FDTS
-
Bacillus anthracis
2.1.1.148 FDTS
-
Paramecium bursaria Chlorella virus-1
2.1.1.148 FDTS
-
Treponema palladium
2.1.1.148 FDTS
-
Mycobacterium tuberculosis
2.1.1.148 flavin-dependent thymidylate synthase
-
Chlamydia trachomatis
2.1.1.148 flavin-dependent thymidylate synthase
-
Pyrococcus furiosus
2.1.1.148 flavin-dependent thymidylate synthase
-
Mycobacterium leprae
2.1.1.148 flavin-dependent thymidylate synthase
-
Helicobacter pylori
2.1.1.148 flavin-dependent thymidylate synthase
-
Thermotoga maritima
2.1.1.148 flavin-dependent thymidylate synthase
-
Clostridium botulinum
2.1.1.148 flavin-dependent thymidylate synthase
-
Rickettsia sp.
2.1.1.148 flavin-dependent thymidylate synthase
-
Bacillus anthracis
2.1.1.148 flavin-dependent thymidylate synthase
-
Paramecium bursaria Chlorella virus-1
2.1.1.148 flavin-dependent thymidylate synthase
-
Treponema palladium
2.1.1.148 flavin-dependent thymidylate synthase
-
Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.74 FADH2
-
Geobacillus stearothermophilus
2.1.1.74 FADH2
-
Mycobacterium leprae
2.1.1.74 FADH2
-
Mycobacterium tuberculosis
2.1.1.74 FADH2
-
Clostridium acidi-urici
2.1.1.74 FADH2
-
Clostridium botulinum
2.1.1.74 FADH2
-
Rickettsia sp.
2.1.1.74 FADH2
-
Bacillus anthracis
2.1.1.74 FADH2
-
Treponema palladium
2.1.1.148 FAD
-
Helicobacter pylori
2.1.1.148 NADH
-
Helicobacter pylori