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Literature summary extracted from

  • Lu, L.; Zhao, M.; Zhang, B.B.; Yu, S.Y.; Bian, X.J.; Wang, W.; Wang, Y.
    Purification and characterization of laccase from Pycnoporus sanguineus and decolorization of an anthraquinone dye by the enzyme (2007), Appl. Microbiol. Biotechnol., 74, 1232-1239.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 dithiothreitol strong inhibition at 1 mM Trametes sanguinea
1.10.3.2 EDTA slight inhibition Trametes sanguinea
1.10.3.2 L-cysteine strong inhibition at 1 mM Trametes sanguinea
1.10.3.2 Sodium azide strong inhibition at 0.1 mM Trametes sanguinea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.077
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.0, 25°C Trametes sanguinea
1.10.3.2 0.091
-
 syringaldazine pH 5.0, 25°C Trametes sanguinea
1.10.3.2 0.203
-
 2,6-dimethoxyphenol pH 4.0, 25°C Trametes sanguinea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 Cu2+ a multicopper oxidase Trametes sanguinea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 61400
-
 x * 61400, SDS-PAGE Trametes sanguinea

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Trametes sanguinea
-
 a white rot fungus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2 native enzyme 3.61fold by ultrafiltration, anion exchange chromatography, and gel filtration Trametes sanguinea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.2 additional information cultured on potato dextrose agar Trametes sanguinea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.2 additional information
-
-
 Trametes sanguinea
1.10.3.2 340.8
-
 purified enzyme Trametes sanguinea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Trametes sanguinea ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2 preferred substrate Trametes sanguinea 3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O i.e. coerulignone ?
1.10.3.2 additional information decolorization of an anthraquinone dye by the enzyme, best at pH 3.0 and 50°C, overview Trametes sanguinea ?
-
 ?
1.10.3.2 syringaldazine + O2
-
 Trametes sanguinea ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 ? x * 61400, SDS-PAGE Trametes sanguinea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 65
-
-
 Trametes sanguinea

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 40
-
 purified enzyme, stable up to Trametes sanguinea
1.10.3.2 50
-
 purified enzyme, rapid decrease in activity Trametes sanguinea

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 0.26
-
 syringaldazine pH 5.0, 25°C Trametes sanguinea
1.10.3.2 6.91
-
 2,6-dimethoxyphenol pH 4.0, 25°C Trametes sanguinea
1.10.3.2 51.52
-
 syringaldazine pH 5.0, 25°C Trametes sanguinea
1.10.3.2 68.87
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.0, 25°C Trametes sanguinea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 3 5 dependent on the substrate Trametes sanguinea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.10.3.2 2 5 the purified enzyme is highly active within this range Trametes sanguinea