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Literature summary extracted from

  • Hamamatsu, N.; Suzumura, A.; Nomiya, Y.; Sato, M.; Aita, T.; Nakajima, M.; Husimi, Y.; Shibanaka, Y.
    Modified substrate specificity of pyrroloquinoline quinone glucose dehydrogenase by biased mutation assembling with optimized amino acid substitution (2006), Appl. Microbiol. Biotechnol., 73, 607-617.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.5.2 into a His-tagged vector for expression in Escherichia coli JM109 Acinetobacter calcoaceticus

Protein Variants

EC Number Protein Variants Comment Organism
1.1.5.2 A71P/N454S mutant, relative activity vs wild type, substrate glucose 0.95, substrate maltose 0.75 Acinetobacter calcoaceticus
1.1.5.2 A98G/K126R/L445I/N454S mutant, relative activity vs wild type, substrate glucose 1.00, substrate maltose 0.78 Acinetobacter calcoaceticus
1.1.5.2 G100R mutant, relative activity vs wild type, substrate glucose 0.35, substrate maltose 0.26 Acinetobacter calcoaceticus
1.1.5.2 G100W/G320E/M367P/A376T mutant, relative activity vs wild type, substrate glucose 0.55, substrate maltose 0.20 Acinetobacter calcoaceticus
1.1.5.2 G320D/M367P/A376T mutant, relative activity vs wild type, substrate glucose 0.51, substrate maltose 0.16 Acinetobacter calcoaceticus
1.1.5.2 G320D/M367P/A376T mutant, relative activity vs wild type, substrate glucose 0.69, substrate maltose 0.24 Acinetobacter calcoaceticus
1.1.5.2 G320E mutant, relative activity vs wild type, substrate glucose 0.92, substrate maltose 0.70 Acinetobacter calcoaceticus
1.1.5.2 G320E/M367P/A376T mutant, relative activity vs wild type, substrate glucose 0.69, substrate maltose 0.25 Acinetobacter calcoaceticus
1.1.5.2 G320F/M367P/A376T mutant, relative activity vs wild type, substrate glucose 0.48, substrate maltose 0.17 Acinetobacter calcoaceticus
1.1.5.2 G320Y/M367P/A376T mutant, relative activity vs wild type, substrate glucose 0.49, substrate maltose 0.16 Acinetobacter calcoaceticus
1.1.5.2 K3E/E278G/G392C mutant, relative activity vs wild type, substrate glucose 0.92, substrate maltose 0.53 Acinetobacter calcoaceticus
1.1.5.2 L194F/A376T mutant, relative activity vs wild type, substrate glucose 0.39, substrate maltose 0.075 Acinetobacter calcoaceticus
1.1.5.2 L194F/G320E/M367P mutant, relative activity vs wild type, substrate glucose 0.38, substrate maltose 0.14 Acinetobacter calcoaceticus
1.1.5.2 L194F/G320E/M367P/A376T mutant, relative activity vs wild type, substrate glucose 0.36, substrate maltose 0.051 Acinetobacter calcoaceticus
1.1.5.2 L194F/G320F mutant, relative activity vs wild type, substrate glucose 0.38, substrate maltose 0.060 Acinetobacter calcoaceticus
1.1.5.2 L194Q mutant, relative activity vs wild type, substrate glucose 0.22, substrate maltose 0.15 Acinetobacter calcoaceticus
1.1.5.2 M367P/A376T mutant, relative activity vs wild type, substrate glucose 0.65, substrate maltose 0.24 Acinetobacter calcoaceticus
1.1.5.2 N454S mutant, relative activity vs wild type, substrate glucose 0.87, substrate maltose 0.69 Acinetobacter calcoaceticus
1.1.5.2 Q193H mutant, relative activity vs wild type, substrate glucose 0.41, substrate maltose 0.23 Acinetobacter calcoaceticus
1.1.5.2 Q193S/G320E mutant, relative activity vs wild type, substrate glucose 0.56, substrate maltose 0.19 Acinetobacter calcoaceticus
1.1.5.2 V157I/M367V/T463S mutant, relative activity vs wild type, substrate glucose 1.00, substrate maltose 0.78 Acinetobacter calcoaceticus
1.1.5.2 V91A/W372R mutant, relative activity vs wild type, substrate glucose 0.44, substrate maltose 0.22 Acinetobacter calcoaceticus
1.1.5.2 Y248F/N342D/A376T/A418V mutant, relative activity vs wild type, substrate glucose 0.74, substrate maltose 0.43 Acinetobacter calcoaceticus
1.1.5.2 Y302H mutant, relative activity vs wild type, substrate glucose 0.47, substrate maltose 0.35 Acinetobacter calcoaceticus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.5.2 Ca2+
-
 Acinetobacter calcoaceticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.5.2 50000
-
 monomer Acinetobacter calcoaceticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.5.2 D-glucose + ubiquinone Acinetobacter calcoaceticus
-
 D-glucono-1,5-lactone + ubiquinol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.2 Acinetobacter calcoaceticus P13650 precursor
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.5.2 using a His Microspin purification module Acinetobacter calcoaceticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.2 D-galactose + ubiquinone
-
 Acinetobacter calcoaceticus ?
-
 ?
1.1.5.2 D-glucose + ubiquinone
-
 Acinetobacter calcoaceticus D-glucono-1,5-lactone + ubiquinol
-
 ?
1.1.5.2 lactose + ubiquinone
-
 Acinetobacter calcoaceticus ?
-
 ?
1.1.5.2 maltose + ubiquinone
-
 Acinetobacter calcoaceticus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.5.2 homodimer 2 * 50000 Da Acinetobacter calcoaceticus

Synonyms

EC Number Synonyms Comment Organism
1.1.5.2 PQQ glucose dehydrogenase
-
 Acinetobacter calcoaceticus
1.1.5.2 PQQGDH-B
-
 Acinetobacter calcoaceticus
1.1.5.2 quinoprotein glucose dehydrogenase
-
 Acinetobacter calcoaceticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.5.2 7
-
 activity assay Acinetobacter calcoaceticus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.5.2 pyrroloquinoline quinone
-
 Acinetobacter calcoaceticus