EC Number | Cloned (Comment) | Organism |
---|---|---|
1.5.3.1 | - |
Bacillus sp. (in: Bacteria) |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.3.1 | 3.1 | - |
sarcosine | pH 8.5, 60°C | Bacillus sp. (in: Bacteria) |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.5.3.1 | 43000 | - |
x * 43000, calculated, x * 51000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
1.5.3.1 | 51000 | - |
x * 43000, calculated, x * 51000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.3.1 | Bacillus sp. (in: Bacteria) | Q6ITC6 | expression in Escherichia coli | - |
1.5.3.1 | Bacillus sp. (in: Bacteria) BSD-8 | Q6ITC6 | expression in Escherichia coli | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.3.1 | recombinant protein | Bacillus sp. (in: Bacteria) |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.3.1 | sarcosine + H2O + O2 | - |
Bacillus sp. (in: Bacteria) | glycine + formaldehyde + H2O2 | - |
? | |
1.5.3.1 | sarcosine + H2O + O2 | - |
Bacillus sp. (in: Bacteria) BSD-8 | glycine + formaldehyde + H2O2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.5.3.1 | ? | x * 43000, calculated, x * 51000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.3.1 | 60 | - |
- |
Bacillus sp. (in: Bacteria) |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.3.1 | 60 | - |
stable | Bacillus sp. (in: Bacteria) |
1.5.3.1 | 65 | - |
10 min, complete loss of activity | Bacillus sp. (in: Bacteria) |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.3.1 | 20.3 | - |
sarcosine | pH 8.5, 60°C | Bacillus sp. (in: Bacteria) |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.5.3.1 | 8.5 | - |
- |
Bacillus sp. (in: Bacteria) |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.5.3.1 | 7 | 10 | - |
Bacillus sp. (in: Bacteria) |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.5.3.1 | 6.5 | 11 | - |
Bacillus sp. (in: Bacteria) |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.3.1 | FAD | wild-type enzyme, noncovalently bound, recombinant enzyme, covalently bound | Bacillus sp. (in: Bacteria) |