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Literature summary extracted from

  • Wen, L.; Huang, H.M.; Juang, R.H.; Lin, C.T.
    Biochemical characterization of 1-Cys peroxiredoxin from Antrodia camphorata (2007), Appl. Microbiol. Biotechnol., 73, 1314-1322.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.24 expression in Escherichia coli Taiwanofungus camphoratus

General Stability

EC Number General Stability Organism
1.11.1.24 1.6 M imidazole seems to promote protein degradation Taiwanofungus camphoratus
1.11.1.24 sensitive to trypsin and chymotrypsin treatment. Very little or no activity detected after 40 min incubation with either protease Taiwanofungus camphoratus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.24 imidazole approximately 70% of the Prx activity is lost in the presence of 0.4 M imidazole or higher. The presence of 1.6 M imidazole seems to promote protein degradation Taiwanofungus camphoratus
1.11.1.24 SDS activity is lost completely in the presence of 1% SDS or higher Taiwanofungus camphoratus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.24 25000
-
 x * 25000, SDS-PAGE Taiwanofungus camphoratus
1.11.1.24 25081
-
 x * 25081, calculated from sequence Taiwanofungus camphoratus

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.24 Taiwanofungus camphoratus A1KZ88
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.24
-
 Taiwanofungus camphoratus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.1.24 fruit body
-
 Taiwanofungus camphoratus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.24 tert-butyl hydroperoxide + reduced dithiothreitol
-
 Taiwanofungus camphoratus tert-butanol + oxidized dithiothreitol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.24 ? x * 25000, SDS-PAGE Taiwanofungus camphoratus
1.11.1.24 ? x * 25081, calculated from sequence Taiwanofungus camphoratus

Synonyms

EC Number Synonyms Comment Organism
1.11.1.24 1-Cys peroxiredoxin
-
 Taiwanofungus camphoratus
1.11.1.24 Ac-1-Cys Prx
-
 Taiwanofungus camphoratus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.24 60
-
 half-life: 15.5 min Taiwanofungus camphoratus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.24 9
-
-
 Taiwanofungus camphoratus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.11.1.24 5.4 9 no activity at or below pH 5.4, optimal activity at pH 9.0 Taiwanofungus camphoratus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.11.1.24 7.8 10.2 37°C, 30 min, stable Taiwanofungus camphoratus