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Literature summary extracted from

  • Jaureguibeitia, A.; Saa, L.; Llama, M.J.; Serra, J.L.
    Purification, characterization and cloning of aldehyde dehydrogenase from Rhodococcus erythropolis UPV-1 (2007), Appl. Microbiol. Biotechnol., 73, 1073-1086.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.3 when expressed in Escherichia coli M15 most of the protein is localised in inclusion bodies, not used for the enzyme assays Rhodococcus erythropolis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.3 1-propanol 7% inhibition at 1 mM Rhodococcus erythropolis
1.2.1.3 EDTA 10-20% inhibition at 1 mM Rhodococcus erythropolis
1.2.1.3 iodacetamide 10-20% inhibition at 1 mM Rhodococcus erythropolis
1.2.1.3 KCN 10-20% inhibition at 1 mM Rhodococcus erythropolis
1.2.1.3 methanol 17% inhibition at 1 mM Rhodococcus erythropolis
1.2.1.3 Ni2+ 52% inhibition at 1 mM Rhodococcus erythropolis
1.2.1.3 p-hydroxymercuribenzoate complete inhibition at 0.1 mM Rhodococcus erythropolis
1.2.1.3 Zn2+ 73% inhibition at 1 mM Rhodococcus erythropolis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.3 0.000085
-
 octanal 1 mM NAD+, 0,05 mM substrate, 250 mM sodium bicarbonate-NaOH buffer pH 10.0, 37°C Rhodococcus erythropolis
1.2.1.3 0.0018
-
 hexanal 1 mM NAD+, 0,05 mM substrate, 250 mM sodium bicarbonate-NaOH buffer pH 10.0, 37°C Rhodococcus erythropolis
1.2.1.3 0.0038
-
 Butanal 1 mM NAD+, 0,05 mM substrate, 250 mM sodium bicarbonate-NaOH buffer pH 10.0, 37°C Rhodococcus erythropolis
1.2.1.3 0.0091
-
 propanal 1 mM NAD+, 0,05 mM substrate, 250 mM sodium bicarbonate-NaOH buffer pH 10.0, 37°C Rhodococcus erythropolis
1.2.1.3 0.09
-
 NAD+ 0,05 mM propanal, 250 mM sodium bicarbonate-NaOH buffer pH 10.0, 37°C Rhodococcus erythropolis
1.2.1.3 0.333
-
 formaldehyde 1 mM NAD+, 0,05 mM substrate, 250 mM sodium bicarbonate-NaOH buffer pH 10.0, 37°C Rhodococcus erythropolis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.3 Mg2+ 23% activation at 1 mM Rhodococcus erythropolis
1.2.1.3 NH4+ 13% activation at 1 mM Rhodococcus erythropolis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.3 16200
-
 non-denaturing PAGE Rhodococcus erythropolis
1.2.1.3 54000
-
 3 * 54000, non-denaturing PAGE Rhodococcus erythropolis
1.2.1.3 54680
-
 MALDI-TOF MS Rhodococcus erythropolis

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.3 Rhodococcus erythropolis Q4F895
-
-
1.2.1.3 Rhodococcus erythropolis UPV-1 Q4F895
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.3 two chromatography steps to homogeneity Rhodococcus erythropolis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.3 cell culture
-
 Rhodococcus erythropolis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.3 19.5
-
-
 Rhodococcus erythropolis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.3 butanal + NAD+ + H2O
-
 Rhodococcus erythropolis butyrate + NADH + H+
-
 ?
1.2.1.3 butanal + NAD+ + H2O
-
 Rhodococcus erythropolis UPV-1 butyrate + NADH + H+
-
 ?
1.2.1.3 formaldehyde + NAD+ + H2O
-
 Rhodococcus erythropolis formate + NADH
-
 ?
1.2.1.3 formaldehyde + NAD+ + H2O
-
 Rhodococcus erythropolis UPV-1 formate + NADH
-
 ?
1.2.1.3 hexanal + NAD+ + H2O
-
 Rhodococcus erythropolis hexanoate + NADH + H+
-
 ?
1.2.1.3 hexanal + NAD+ + H2O
-
 Rhodococcus erythropolis UPV-1 hexanoate + NADH + H+
-
 ?
1.2.1.3 octanal + NAD+ + H2O
-
 Rhodococcus erythropolis octanoate + NADH
-
 ?
1.2.1.3 octanal + NAD+ + H2O
-
 Rhodococcus erythropolis UPV-1 octanoate + NADH
-
 ?
1.2.1.3 propanal + NAD+ + H2O
-
 Rhodococcus erythropolis propanoate + NADH + H+
-
 ?
1.2.1.3 propanal + NAD+ + H2O
-
 Rhodococcus erythropolis UPV-1 propanoate + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.3 trimer 3 * 54000, non-denaturing PAGE Rhodococcus erythropolis

Synonyms

EC Number Synonyms Comment Organism
1.2.1.3 aldehyde dehydrogenase
-
 Rhodococcus erythropolis
1.2.1.3 ALDH
-
 Rhodococcus erythropolis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.3 47.5
-
 with n-propanal as substrate Rhodococcus erythropolis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.3 30
-
 fully stable after 10 min Rhodococcus erythropolis
1.2.1.3 40
-
 75% activity after 10 min in presence or absence of substrates Rhodococcus erythropolis
1.2.1.3 40
-
 fully active after 10 min when incubated with NAD+ Rhodococcus erythropolis
1.2.1.3 60
-
 completely inactive after 10 min Rhodococcus erythropolis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.3 9.5
-
 with n-propanal as substrate Rhodococcus erythropolis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.2.1.3 7 9 60% activity after 16 h at 4°C Rhodococcus erythropolis
1.2.1.3 9.5
-
 10% activity when stored at a pH above 9.5 Rhodococcus erythropolis

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.3 NAD+ totally inactive with NADP+ Rhodococcus erythropolis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.2.1.3 Rhodococcus erythropolis two-dimensional electrophoresis
-
 5.3