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Literature summary extracted from

  • Salony, T.; Mishra, S.; Bisaria, V.S.
    Production and characterization of laccase from Cyathus bulleri and its use in decolourization of recalcitrant textile dyes (2006), Appl. Microbiol. Biotechnol., 71, 646-653.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.10.3.2 additional information highest induction by 2,6-dimethylaniline, but also by 3,5-dihydroxybenzoic acid, guaiacol, 4-hydroxy-3-methoxycinnamic acid, calcium lignosulphonate, 2,5-dimethylaniline, 3,4-dimethylaniline, 3,4-dimethoxybenzaldehyde, 4-hydroxybenzaldehyde, and 2,6-dihydroxybenzoic acid, overview Cyathus bulleri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 58000
-
 gel filtration Cyathus bulleri

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Cyathus bulleri
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.10.3.2 glycoprotein 16% carbohydrate content Cyathus bulleri

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2 native enzyme 82fold from the culture filtrate after 7 days of growth of the mycelium, by ultrafiltration, anion exchange chromatography, and gel filtration Cyathus bulleri

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.2 mycelium
-
 Cyathus bulleri
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.2 4022
-
 purified enzyme Cyathus bulleri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Cyathus bulleri ?
-
 ?
1.10.3.2 4-hydroquinone + O2
-
 Cyathus bulleri ?
-
 ?
1.10.3.2 guaiacol + O2 best substrate Cyathus bulleri ?
-
 ?
1.10.3.2 pyrogallol + O2
-
 Cyathus bulleri ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 monomer 1 * 58000, SDS-PAGE Cyathus bulleri

Synonyms

EC Number Synonyms Comment Organism
1.10.3.2 p-diphenol:oxygen oxidoreductase
-
 Cyathus bulleri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 30
-
-
 Cyathus bulleri

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 35
-
 half-life: 63 min Cyathus bulleri
1.10.3.2 40
-
 half-life: 48 min Cyathus bulleri
1.10.3.2 45
-
 half-life: 36 min Cyathus bulleri
1.10.3.2 50
-
 half-life: 18 min Cyathus bulleri
1.10.3.2 55
-
 loss of 80% activity, half-life: 12 min Cyathus bulleri
1.10.3.2 60
-
 complete inactivation, half-life: 4 min Cyathus bulleri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 5.5
-
-
 Cyathus bulleri

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.10.3.2 3.5 9
-
 Cyathus bulleri