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Literature summary extracted from

  • Hofrichter, M.; Ullrich, R.
    Heme-thiolate haloperoxidases: versatile biocatalysts with biotechnological and environmental significance (2006), Appl. Microbiol. Biotechnol., 71, 276-288.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.10 expression in Aspergillus niger Leptoxyphium fumago

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.10 42000
-
-
 Leptoxyphium fumago
1.11.2.1 46000
-
 gel filtration Cyclocybe aegerita

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.10 Leptoxyphium fumago
-
-
-
1.11.1.18 Cyclocybe aegerita
-
-
-
1.11.2.1 Cyclocybe aegerita
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.11.1.10 glycoprotein heavily glycosylated protein (25–30% carbohydrates, two high-mannose N-glycosylation sites) Leptoxyphium fumago
1.11.2.1 glycoprotein
-
 Cyclocybe aegerita

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.2.1
-
 Cyclocybe aegerita

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.10 additional information catalyzes the unspecific chlorination, bromination, and iodation (but no fluorination) of a variety of electrophilic organic substrates via hypohalous acid as actual halogenating agent. In the absence of halide, CPO resembles cytochrome P450s and epoxidizes and hydroxylates activated substrates such as organic sulfides and olefins. Aromatic rings are not susceptible to CPO-catalyzed oxygen-transfer Leptoxyphium fumago ?
-
 ?
1.11.1.18 additional information strong brominating aactivity, weak chlorinating and iodating activities, catalyzes both benzylic and aromatic hydroxylations (e.g., of toluene and naphthalene) Cyclocybe aegerita ?
-
 ?
1.11.1.18 phenol + H2O2 + Br-
-
 Cyclocybe aegerita 4-bromophenol + 2-bromophenol + H2O 4-bromophenol + 2-bromophenol at the ratio of 4:1 ?
1.11.2.1 4-ethoxy-3-methoxybenzyl alcohol + H2O2
-
 Cyclocybe aegerita 4-ethoxy-3-methoxybenzaldehyde + H2O
-
 ?
1.11.2.1 anisyl alcohol + H2O2
-
 Cyclocybe aegerita anisaldehyde + H2O
-
 ?
1.11.2.1 benzyl alcohol + H2O2
-
 Cyclocybe aegerita benzaldehyde + H2O
-
 ?
1.11.2.1 additional information the Agrocybe aegerita peroxidase has strong brominating as well as weak chlorinating and iodating activities, and catalyzes both benzylic and aromatic hydroxylations Cyclocybe aegerita ?
-
 ?
1.11.2.1 naphthalene + H2O2
-
 Cyclocybe aegerita ?
-
 ?
1.11.2.1 phenol + KBr the Agrocybe aegerita peroxidase has also strong brominating activity Cyclocybe aegerita 4-bromophenol + 2-bromophenol
-
 ?
1.11.2.1 toluene + H2O2
-
 Cyclocybe aegerita 4-cresol + 2-cresol + methyl-4-benzoquinone + H2O
-
 ?
1.11.2.1 vanillyl alcohol + H2O2
-
 Cyclocybe aegerita vanillaldehyde + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.10 CPO
-
 Leptoxyphium fumago
1.11.1.10 heme-thiolate chloroperoxidase
-
 Leptoxyphium fumago
1.11.2.1 Agrocybe aegerita peroxidase
-
 Cyclocybe aegerita

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.10 heme
-
 Leptoxyphium fumago
1.11.2.1 heme
-
 Cyclocybe aegerita

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.11.1.10 Leptoxyphium fumago
-
-
 4
1.11.2.1 Cyclocybe aegerita there are six isoforms with different isoelectric points between pH 4.6 and 5.4, isoelectric focusing 5.4 4.6

Expression

EC Number Organism Comment Expression
1.11.2.1 Cyclocybe aegerita Agrocybe aegerita produces relatively high and stable peroxidase levels in complex liquid media based on soybeans up