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Literature summary extracted from

  • Michniewicz, A.; Ullrich, R.; Ledakowicz, S.; Hofrichter, M.
    The white-rot fungus Cerrena unicolor strain 137 produces two laccase isoforms with different physico-chemical and catalytic properties (2006), Appl. Microbiol. Biotechnol., 69, 682-688.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 Cu2+ required, stimulates up to 0.2 mM, contains type I Cu+ and type III Cu+ Cerrena unicolor

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 57000
-
 1 * 64000, isozyme Lacc I, SDS-PAGE, 1 * 57000, isozyme Lacc II, SDS-PAGE Cerrena unicolor
1.10.3.2 64000
-
 1 * 64000, isozyme Lacc I, SDS-PAGE, 1 * 57000, isozyme Lacc II, SDS-PAGE Cerrena unicolor

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Cerrena unicolor
-
 two isozymes Lacc I and Lacc II
-
1.10.3.2 Cerrena unicolor 137
-
 two isozymes Lacc I and Lacc II
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2 native enzyme 26fold by ultrafiltration/diafiltration, gel filtration and anion exchange chromatography Cerrena unicolor

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.2 additional information high production rate culture, overview Cerrena unicolor
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.2 additional information
-
-
 Cerrena unicolor
1.10.3.2 206
-
 purified enzyme Cerrena unicolor

Storage Stability

EC Number Storage Stability Organism
1.10.3.2 4°C, purified isozymes in 10 mM Na acetate buffer, pH 5.0, isozyme Lacc II is stable for at least 6 months, isozyme Lacc I shows 40% remaining activity after 6 months Cerrena unicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Cerrena unicolor ?
-
 ?
1.10.3.2 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Cerrena unicolor 137 ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Cerrena unicolor 3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Cerrena unicolor 137 3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O
-
 ?
1.10.3.2 syringaldazine + O2
-
 Cerrena unicolor ?
-
 ?
1.10.3.2 syringaldazine + O2
-
 Cerrena unicolor 137 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 monomer 1 * 64000, isozyme Lacc I, SDS-PAGE, 1 * 57000, isozyme Lacc II, SDS-PAGE Cerrena unicolor

Synonyms

EC Number Synonyms Comment Organism
1.10.3.2 Lacc
-
 Cerrena unicolor

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 25
-
 assay at Cerrena unicolor

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.10.3.2 5 75 high activity at 5°C, 25 and 30% of maximum activity at 60°C for isozymes Lacc I and II, 20% at 75°C Cerrena unicolor

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 25
-
 purified isozymes Lacc I and Lacc II are stable Cerrena unicolor
1.10.3.2 50
-
 60 min, purified isozyme Lacc I shows loss of 20% activity, purified isozyme Lacc II is stable Cerrena unicolor
1.10.3.2 70
-
 20 min, purified isozyme Lacc I shows complete loss of activity, isozyme Lacc II 10% after 60 min Cerrena unicolor
1.10.3.2 80
-
 rapid inactivation of purified isozyme Lacc II with 95% loss of activity within 10 min Cerrena unicolor

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 3.5 5.5 dependent on the substrate Cerrena unicolor

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.10.3.2 2.5 5.5 inactivation of isozyme Lacc I at pH 6.5, of isozyme Lacc II at pH 7.5 Cerrena unicolor

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.2 3
-
 partial inactivation of both isozymes, 60-64% remaining activity after 1 h Cerrena unicolor
1.10.3.2 7
-
 isozyme Lacc I and isozyme Lacc II, stable Cerrena unicolor
1.10.3.2 10
-
 isozyme Lacc II is stable, isozyme Lacc I loses 10% of activity within 1 h Cerrena unicolor

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.10.3.2 Cerrena unicolor isozyme Lacc I, isoelectric focusing
-
 3.6
1.10.3.2 Cerrena unicolor isozyme Lacc II, isoelectric focusing
-
 3.7