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Literature summary extracted from

  • Ohmachi, T.; Narita, M.; Kawata, M.; Bizen, A.; Tamura, Y.; Asada, Y.
    A novel N-carbamoyl-L-amino acid amidohydrolase of Pseudomonas sp. strain ON-4a: purification and characterization of N-carbamoyl-L-cysteine amidohydrolase expressed in Escherichia coli (2004), Appl. Microbiol. Biotechnol., 65, 686-693.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.87 Mg2+
-
 Pseudomonas sp.
3.5.1.87 Zn2+
-
 Pseudomonas sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.87 NCC amidohydrolase gene is cloned into DH5alpha and pet15b vectors for protein expression in Escherichia coli Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.87 EDTA
-
 Pseudomonas sp.
3.5.1.87 Hg2+
-
 Pseudomonas sp.
3.5.1.87 iodoacetic acid IAA Pseudomonas sp.
3.5.1.87 N-ethyl-maleimide NEM Pseudomonas sp.
3.5.1.87 p-chloromercuribenzoic acid PCMB Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.87 Mn2+
-
 Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.87 45000
-
 monomer, 12,5% SDS-PAGE Pseudomonas sp.
3.5.1.87 170000
-
 native enzyme, tetramer, estimated by Sephacryl S-300 gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.87 N-carbamoyl-L-cysteine + H2O Pseudomonas sp.
-
 L-cysteine + CO2 + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.87 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.87 anion-exchange chromatography, hydrophobic interaction chromatography hydroxyapatite chromatography used for purification of native expressed protein Pseudomonas sp.

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.5.1.87 protein from inclusion bodies is solubilized, recovered and purified by metal affinity chromatography Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.87 15.9
-
 protein recovered from inclusion bodies Pseudomonas sp.
3.5.1.87 23.2
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.87 N-carbamoyl-DL-aspartic acid + H2O relative activity 7.81% Pseudomonas sp. DL-aspartic acid + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-DL-cysteine + H2O relative activity 119% Pseudomonas sp. DL-cysteine + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-DL-serine + H2O relative activity 37.8% Pseudomonas sp. DL-serine + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-glycine + H2O relative activity 72.4% Pseudomonas sp. glycine + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-L-alanine + H2O relative activity 41.2% Pseudomonas sp. L-alanine + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-L-cysteine + H2O
-
 Pseudomonas sp. L-cysteine + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-L-cysteine + H2O relative activity 100% Pseudomonas sp. L-cysteine + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-L-methionine + H2O relative activity 44.4% Pseudomonas sp. L-methionine + CO2 + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.87 tetramer
-
 Pseudomonas sp.

Synonyms

EC Number Synonyms Comment Organism
3.5.1.87 N-carbamoyl-L-amino-acid amidohydrolase
-
 Pseudomonas sp.
3.5.1.87 N-carbamoyl-L-amino-acid hydrolase
-
 Pseudomonas sp.
3.5.1.87 N-carbamoyl-L-cysteine-acid amidohydrolase
-
 Pseudomonas sp.
3.5.1.87 NCC amidohydrolase
-
 Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.87 50
-
 activity assay Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.87 9
-
 activity assay Pseudomonas sp.