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Literature summary extracted from

  • Takimoto, A.; Takakura, T.; Tani, H.; Yagi, S.; Mitsushima, K.
    Batch production of deacetyl 7-aminocephalosporanic acid by immobilized cephalosporin-C deacetylase (2004), Appl. Microbiol. Biotechnol., 65, 263-267.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.1.41 synthesis production of deacetyl 7-aminocephalosporanic acid as precursor for the synthesis of cefcapene pivoxil hydrochloride (Flomox) Bacillus subtilis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.41 expressed in Escherichia coli JM109 Bacillus subtilis

General Stability

EC Number General Stability Organism
3.1.1.41 immobilized enzyme is very stable and exhibited only a slight decrease in activity upon repeated use Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.41 additional information no inhibition by reaction products Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.41 cephalosporin C + H2O Bacillus subtilis
-
 deacetylcephalosporin C + acetate
-
 ?
3.1.1.41 cephalosporin C + H2O Bacillus subtilis SHS0133
-
 deacetylcephalosporin C + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.41 Bacillus subtilis
-
 recombinant enzyme from Escherichia coli immobilized on an anion exchange resin
-
3.1.1.41 Bacillus subtilis SHS0133
-
 recombinant enzyme from Escherichia coli immobilized on an anion exchange resin
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.41 recombinant protein purified to 90% Bacillus subtilis

Storage Stability

EC Number Storage Stability Organism
3.1.1.41 5°C, pH 8.0, immobilized enzyme, 2 years, 4% loss of activity Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.41 7-aminocephalosporanic acid + H2O
-
 Bacillus subtilis deacetyl 7-aminocephalosporanic acid + acetate reaction product is not less than 97.5% pure, only a negligible amount of deacetyl 7-aminocephalospornolactone detected ?
3.1.1.41 7-aminocephalosporanic acid + H2O
-
 Bacillus subtilis SHS0133 deacetyl 7-aminocephalosporanic acid + acetate reaction product is not less than 97.5% pure, only a negligible amount of deacetyl 7-aminocephalospornolactone detected ?
3.1.1.41 cephalosporin C + H2O
-
 Bacillus subtilis deacetylcephalosporin C + acetate
-
 ?
3.1.1.41 cephalosporin C + H2O
-
 Bacillus subtilis SHS0133 deacetylcephalosporin C + acetate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.41 50
-
 immobilized and free enzyme fully stable Bacillus subtilis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.41 4 11 immobilized enzyme stable for at least 60 min at 20°C Bacillus subtilis
3.1.1.41 10
-
 free enzyme shows highest activity after incubation at pH10 for 60 min at 20°C, about 15% of activity remaining after incubation at pH4 Bacillus subtilis