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Literature summary extracted from

  • Sakasegawa, S.; Ishikawa, H.; Imamura, S.; Sakuraba, H.; Goda, S.; Ohshima, T.
    Bilirubin oxidase activity of Bacillus subtilis CotA (2006), Appl. Environ. Microbiol., 72, 972-975.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.3.3.5 medicine great potential for use as a diagnostic reagent, can be used for accurate determination of serum bilirubin levels in patients with hepatobiliary disease Bacillus subtilis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.3.5 Escherichia coli JM109 transformed with pUC119 carrying the CotA gene, produces large amounts of the soluble protein under low-temperature conditions Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.3.5 0.008
-
 bilirubin
-
 Bacillus subtilis
1.3.3.5 0.015
-
 ditaurobilirubin
-
 Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.3.5 Cu2+
-
 Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.5 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.3.5 by gel filtration Bacillus subtilis

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.3.5 bilirubin + O2 CotA with markedly higher affinity for bilirubin than conventional bilirubin oxidase Bacillus subtilis biliverdin + H2O
-
 ?
1.3.3.5 ditaurobilirubin + O2
-
 Bacillus subtilis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.3.5 CotA
-
 Bacillus subtilis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.3.3.5 21 37 at 24°C approximately 50% of the CotA protein is detected in the soluble fraction Bacillus subtilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.3.3.5 65
-
 stable for 60 min Bacillus subtilis
1.3.3.5 84
-
 stable for 30 min Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.3.5 4
-
 optimal for ditaurobilirubin oxidation Bacillus subtilis
1.3.3.5 7
-
 optimal for bilirubin oxidation Bacillus subtilis