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Literature summary extracted from

  • Laloknam, S.; Tanaka, K.; Buaboocha, T.; Waditee, R.; Incharoensakdi, A.; Hibino, T.; Tanaka, Y.; Takabe, T.
    Halotolerant cyanobacterium Aphanothece halophytica contains a betaine transporter active at alkaline pH and high salinity (2006), Appl. Environ. Microbiol., 72, 6018-6026.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
7.6.2.9 additional information sucrose and sorbitol show no effect Aphanothece halophytica

Application

EC Number Application Comment Organism
7.6.2.9 additional information Na+-betaine symporter that contributes to the salt stress tolerance at alkaline pH Aphanothece halophytica

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.6.2.9 cloned into pApBetT, transfered to Escherichia coli and then to MKH13 cells, overexpression in freshwater Synechococcus sp. strain PCC7942 cells Aphanothece halophytica

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.6.2.9 Betaine aldehyde weakly inhibits betaine uptake Aphanothece halophytica
7.6.2.9 choline slightly inhibits betaine uptake Aphanothece halophytica
7.6.2.9 additional information betaine uptake not inhibited by gamma-aminobutyric acid, proline, glutamate, aspartate, glutamine, asparagine, glycine, sarcosine, dimethylglycine, lysine, histidine, alanine, leucine, isoleucine, serine, cysteine, threonine, valine, phenylalanine, tryptophan, and methionine Aphanothece halophytica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.6.2.9 0.00832
-
 betaine Aphanothece halophytica cells supplemented with 0.5 M NaCl Aphanothece halophytica
7.6.2.9 0.00941
-
 betaine Aphanothece halophytica cells supplemented with 2.0 M NaCl Aphanothece halophytica
7.6.2.9 0.0608
-
 betaine at pH 8.5, Aphanothece halophytica cells Aphanothece halophytica
7.6.2.9 0.0834
-
 betaine at pH 7.0, Aphanothece halophytica cells Aphanothece halophytica
7.6.2.9 0.0919
-
 betaine at pH 8.5, BetTA.halophytica expressing MKH13 cells Aphanothece halophytica
7.6.2.9 0.128
-
 betaine at pH 7.0, BetTA.halophytica expressing MKH13 cells Aphanothece halophytica
7.6.2.9 0.143
-
 betaine at pH 5.5, Aphanothece halophytica cells Aphanothece halophytica
7.6.2.9 0.272
-
 betaine at pH 5.5, BetTA.halophytica expressing MKH13 cells Aphanothece halophytica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.6.2.9 additional information K+, Li+, Cs+, Rb+, NH4+, Ca2+, and Mg2+ show no effect Aphanothece halophytica
7.6.2.9 Na+ enhances betaine uptake rates Aphanothece halophytica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.6.2.9 64660
-
 calculated from sequence analysis Aphanothece halophytica

Organism

EC Number Organism UniProt Comment Textmining
7.6.2.9 Aphanothece halophytica Q0PCR9
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.6.2.9 ATP + H2O + betaine/out
-
 Aphanothece halophytica ADP + phosphate + betaine/in
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.6.2.9 BetTA.halophytica
-
 Aphanothece halophytica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6.2.9 9
-
 for betaine uptake in Aphanothece halophytica cells Aphanothece halophytica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
7.6.2.9 5.5 9
-
 Aphanothece halophytica