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Literature summary extracted from

  • MacLean, A.M.; MacPherson, G.; Aneja, P.; Finan, T.M.
    Characterization of the beta-ketoadipate pathway in Sinorhizobium meliloti (2006), Appl. Environ. Microbiol., 72, 5403-5413.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.3.6 genes SMb20587 and SMb20588, i.e. pcaI and pcaJ organized in an operon with a putative beta-ketoadipyl-CoA thiolase gene pcaF, the expression of the pcaIJF operon is regulated by an IclR-type transcriptional regulator, SMb20586, i.e. pcaR, DNA and amino acid sequence determination and analysis, genetic organization and regulation, overview, subcloning and expression of the enzyme in Escherichia coli Sinorhizobium meliloti

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.3.6 adipate inhibits the enzyme at concentration above 10 mM, activates at 2 mM Sinorhizobium meliloti

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.3.6 Mg2+
-
 Sinorhizobium meliloti

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.3.6 3-oxoadipate + succinyl-CoA Sinorhizobium meliloti
-
 3-oxoadipyl-CoA + succinate
-
 ?
2.8.3.6 additional information Sinorhizobium meliloti the enzyme participates in the beta-ketoadipate pathway, overview, expression of the pcaIJF operon is regulated by an IclR-type transcriptional regulator pcaR, whose transcription is negatively autoregulated, and PcaR is a positive regulator of pcaIJF expression and is required for growth of Sinorhizobium meliloti on protocatechuate as the carbon source, pathway overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.3.6 Sinorhizobium meliloti
-
 a soil-dwelling bacterium, strain Rm1021, genes SMb20587 and SMb20588, i.e. pcaI and pcaJ
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.3.6 3-oxoadipate + succinyl-CoA
-
 Sinorhizobium meliloti 3-oxoadipyl-CoA + succinate
-
 ?
2.8.3.6 additional information the enzyme participates in the beta-ketoadipate pathway, overview, expression of the pcaIJF operon is regulated by an IclR-type transcriptional regulator pcaR, whose transcription is negatively autoregulated, and PcaR is a positive regulator of pcaIJF expression and is required for growth of Sinorhizobium meliloti on protocatechuate as the carbon source, pathway overview Sinorhizobium meliloti ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.8.3.6 beta-ketoadipate succinyl-CoA transferase
-
 Sinorhizobium meliloti
2.8.3.6 beta-ketoadipate succinyl-coenzyme A transferase
-
 Sinorhizobium meliloti

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.3.6 22 23 assay at Sinorhizobium meliloti

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.3.6 8
-
 assay at Sinorhizobium meliloti