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Literature summary extracted from

  • Sasoh, M.; Masai, E.; Ishibashi, S.; Hara, H.; Kamimura, N.; Miyauchi, K.; Fukuda, M.
    Characterization of the terephthalate degradation genes of Comamonas sp. strain E6 (2006), Appl. Environ. Microbiol., 72, 1825-1832.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.12.15 expressed in Escherichia coli BL21 (DE3) cells Comamonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.12.15 EDTA 0.5 mM, complete loss of activity Comamonas sp.
1.14.12.15 EDTA 0.5 mM, complete loss of activity Comamonas testosteroni

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.12.15 Fe2+ required for activity, inhibited TPADO activity is partially recovered only when Fe2+ is added to the reaction mixture Comamonas testosteroni
1.14.12.15 Fe2+ required for activity, inhibited TPADO activity is partially recovered only when Fe2+ is added to the reaction mixture Comamonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.12.15 33000
-
 subunit TphBII, SDS-PAGE Comamonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.15 Comamonas sp.
-
-
-
1.14.12.15 Comamonas sp. E6
-
-
-
1.14.12.15 Comamonas testosteroni
-
-
-
1.14.12.15 Comamonas testosteroni IAM 1152
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.12.15 additional information
-
 in the presence of 1 mM NADPH, the TPA transformation activity of the crude extract measured in a 1-min reaction is 1.3times higher than that with 1 mM NADH Comamonas testosteroni
1.14.12.15 additional information
-
 in the presence of 1 mM NADPH, the TPA transformation activity of the crude extract measured in a 1-min reaction is 1.3times higher than that with 1 mM NADH Comamonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.15 additional information phthalate, isophthalate, benzoate, p-hydroxybenzoate, biphenyl, benzene, and toluene are no substrates of TPADO Comamonas testosteroni ?
-
 ?
1.14.12.15 additional information phthalate, isophthalate, benzoate, p-hydroxybenzoate, biphenyl, benzene, and toluene are no substrates of TPADO Comamonas sp. ?
-
 ?
1.14.12.15 additional information phthalate, isophthalate, benzoate, p-hydroxybenzoate, biphenyl, benzene, and toluene are no substrates of TPADO Comamonas testosteroni IAM 1152 ?
-
 ?
1.14.12.15 additional information phthalate, isophthalate, benzoate, p-hydroxybenzoate, biphenyl, benzene, and toluene are no substrates of TPADO Comamonas sp. E6 ?
-
 ?
1.14.12.15 terephthalate + NAD(P)H + O2 + H+ NADPH is the preferred electron donor compared to NADH Comamonas testosteroni (1R,2S)-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD(P)+
-
 ?
1.14.12.15 terephthalate + NAD(P)H + O2 + H+ NADPH is the preferred electron donor compared to NADH Comamonas sp. (1R,2S)-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD(P)+
-
 ?
1.14.12.15 terephthalate + NAD(P)H + O2 + H+ NADPH is the preferred electron donor compared to NADH Comamonas testosteroni IAM 1152 (1R,2S)-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD(P)+
-
 ?
1.14.12.15 terephthalate + NAD(P)H + O2 + H+ NADPH is the preferred electron donor compared to NADH Comamonas sp. E6 (1R,2S)-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD(P)+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.12.15 TPA 1,2-dioxygenase
-
 Comamonas testosteroni
1.14.12.15 TPA 1,2-dioxygenase
-
 Comamonas sp.
1.14.12.15 TPADO TPADO is strongly suggested to be a two-component dioxygenase which consists of the terminal oxygenase component (TphA2 and TphA3) and the reductase component (TphA1) Comamonas testosteroni
1.14.12.15 TPADO TPADO is strongly suggested to be a two-component dioxygenase which consists of the terminal oxygenase component (TphA2 and TphA3) and the reductase component (TphA1) Comamonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.15 NADPH
-
 Comamonas testosteroni
1.14.12.15 NADPH
-
 Comamonas sp.