EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.12.15 | expressed in Escherichia coli BL21 (DE3) cells | Comamonas sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.12.15 | EDTA | 0.5 mM, complete loss of activity | Comamonas sp. | |
1.14.12.15 | EDTA | 0.5 mM, complete loss of activity | Comamonas testosteroni |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.12.15 | Fe2+ | required for activity, inhibited TPADO activity is partially recovered only when Fe2+ is added to the reaction mixture | Comamonas testosteroni | |
1.14.12.15 | Fe2+ | required for activity, inhibited TPADO activity is partially recovered only when Fe2+ is added to the reaction mixture | Comamonas sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.12.15 | 33000 | - |
subunit TphBII, SDS-PAGE | Comamonas sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.12.15 | Comamonas sp. | - |
- |
- |
1.14.12.15 | Comamonas sp. E6 | - |
- |
- |
1.14.12.15 | Comamonas testosteroni | - |
- |
- |
1.14.12.15 | Comamonas testosteroni IAM 1152 | - |
- |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.12.15 | additional information | - |
in the presence of 1 mM NADPH, the TPA transformation activity of the crude extract measured in a 1-min reaction is 1.3times higher than that with 1 mM NADH | Comamonas testosteroni |
1.14.12.15 | additional information | - |
in the presence of 1 mM NADPH, the TPA transformation activity of the crude extract measured in a 1-min reaction is 1.3times higher than that with 1 mM NADH | Comamonas sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.12.15 | additional information | phthalate, isophthalate, benzoate, p-hydroxybenzoate, biphenyl, benzene, and toluene are no substrates of TPADO | Comamonas testosteroni | ? | - |
? | |
1.14.12.15 | additional information | phthalate, isophthalate, benzoate, p-hydroxybenzoate, biphenyl, benzene, and toluene are no substrates of TPADO | Comamonas sp. | ? | - |
? | |
1.14.12.15 | additional information | phthalate, isophthalate, benzoate, p-hydroxybenzoate, biphenyl, benzene, and toluene are no substrates of TPADO | Comamonas testosteroni IAM 1152 | ? | - |
? | |
1.14.12.15 | additional information | phthalate, isophthalate, benzoate, p-hydroxybenzoate, biphenyl, benzene, and toluene are no substrates of TPADO | Comamonas sp. E6 | ? | - |
? | |
1.14.12.15 | terephthalate + NAD(P)H + O2 + H+ | NADPH is the preferred electron donor compared to NADH | Comamonas testosteroni | (1R,2S)-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD(P)+ | - |
? | |
1.14.12.15 | terephthalate + NAD(P)H + O2 + H+ | NADPH is the preferred electron donor compared to NADH | Comamonas sp. | (1R,2S)-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD(P)+ | - |
? | |
1.14.12.15 | terephthalate + NAD(P)H + O2 + H+ | NADPH is the preferred electron donor compared to NADH | Comamonas testosteroni IAM 1152 | (1R,2S)-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD(P)+ | - |
? | |
1.14.12.15 | terephthalate + NAD(P)H + O2 + H+ | NADPH is the preferred electron donor compared to NADH | Comamonas sp. E6 | (1R,2S)-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD(P)+ | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.12.15 | TPA 1,2-dioxygenase | - |
Comamonas testosteroni |
1.14.12.15 | TPA 1,2-dioxygenase | - |
Comamonas sp. |
1.14.12.15 | TPADO | TPADO is strongly suggested to be a two-component dioxygenase which consists of the terminal oxygenase component (TphA2 and TphA3) and the reductase component (TphA1) | Comamonas testosteroni |
1.14.12.15 | TPADO | TPADO is strongly suggested to be a two-component dioxygenase which consists of the terminal oxygenase component (TphA2 and TphA3) and the reductase component (TphA1) | Comamonas sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.12.15 | NADPH | - |
Comamonas testosteroni | |
1.14.12.15 | NADPH | - |
Comamonas sp. |