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Literature summary extracted from

  • Luetke-Eversloh, T.; Stephanopoulos, G.
    Feedback inhibition of chorismate mutase/prephenate dehydrogenase (TyrA) of Escherichia coli: generation and characterization of tyrosine-insensitive mutants (2005), Appl. Environ. Microbiol., 71, 7224-7228.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.12 mutants cloned into pZE21-MCS1 and transformed to Escherichia coli DH5alpha, wild-type and mutants subcloned into vector pET-30XA/LIC and expressed in Escherichia coli BL21(DE3) Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
1.3.1.12 additional information feedback-inhibition-resistant mutants toward L-tyrosine, chorismate mutase activities similar to those of the wild-type, amino acid substitutions either at Tyr263 or at residues 354 to 357 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.12 L-tyrosine Tyr263 or residues 354 to 357 of the enzyme are involved in the feedback inhibition Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.12 Escherichia coli
-
-
-
1.3.1.12 Escherichia coli K-12 MG1655
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.12 His6-tagged proteins, to homogeneity by gel filtration Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.12 29.8
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.12 prephenate + NAD+
-
 Escherichia coli 4-hydroxyphenylpyruvate + NADH + CO2
-
 ?
1.3.1.12 prephenate + NAD+
-
 Escherichia coli K-12 MG1655 4-hydroxyphenylpyruvate + NADH + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.12 PDH
-
 Escherichia coli
1.3.1.12 tyrA
-
 Escherichia coli