EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.14.1 | gene family Ema, DNA and amino acid sequence determination and analysis | Streptomyces sp. |
1.14.14.1 | gene family ema, DNA and amino acid sequence determination and analysis, overexpression of His-tagged ema1 and ema16 in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli and Streptomyces lividans | Streptomyces tubercidicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.14.1 | additional information | - |
additional information | kinetic analysis of recombinant enzymes | Streptomyces tubercidicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.1 | avermectin + [reduced NADPH-hemoprotein reductase] + O2 | Streptomyces sp. | 4''-oxo-avermectin is a key intermediate in the manufacture of the agriculturally important insecticide emamectin benzoate from the natural product avermectin, overview | 4''-oxo-avermectin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.1 | avermectin + [reduced NADPH-hemoprotein reductase] + O2 | Streptomyces tubercidicus | 4''-oxo-avermectin is a key intermediate in the manufacture of the agriculturally important insecticide emamectin benzoate from the natural product avermectin, overview | 4''-oxo-avermectin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.1 | Streptomyces sp. | - |
several strains, Ema genes | - |
1.14.14.1 | Streptomyces tubercidicus | - |
strains R-922 and I-1529, ema genes, and genes cyp229 and cyp230 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.14.1 | recombinant His-tagged Ema1 and Ema16 from Escherichia coli to near-homogeneity by a two-step purification procedure | Streptomyces tubercidicus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.14.1 | 0.001 | 0.06 | purified recombinant Ema1 and Ema16 proteins | Streptomyces tubercidicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.1 | avermectin + [reduced NADPH-hemoprotein reductase] + O2 | 4''-oxo-avermectin is a key intermediate in the manufacture of the agriculturally important insecticide emamectin benzoate from the natural product avermectin, overview | Streptomyces sp. | 4''-oxo-avermectin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.1 | avermectin + [reduced NADPH-hemoprotein reductase] + O2 | 4''-oxo-avermectin is a key intermediate in the manufacture of the agriculturally important insecticide emamectin benzoate from the natural product avermectin, overview | Streptomyces tubercidicus | 4''-oxo-avermectin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.1 | avermectin + [reduced NADPH-hemoprotein reductase] + O2 | avermectin oxidation activity | Streptomyces sp. | 4''-oxo-avermectin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.1 | avermectin + [reduced NADPH-hemoprotein reductase] + O2 | avermectin oxidation activity | Streptomyces tubercidicus | 4''-oxo-avermectin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.14.1 | Cyp | - |
Streptomyces sp. |
1.14.14.1 | Cyp | - |
Streptomyces tubercidicus |
1.14.14.1 | cytochrome P450 monooxygenase | - |
Streptomyces sp. |
1.14.14.1 | cytochrome P450 monooxygenase | - |
Streptomyces tubercidicus |
1.14.14.1 | Ema | - |
Streptomyces tubercidicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.14.1 | 30 | - |
assya at | Streptomyces tubercidicus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.14.1 | 0.048 | - |
avermectin | pH 7.0, 30°C, recombinant Ema1 | Streptomyces tubercidicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.14.1 | 7 | - |
assay at | Streptomyces tubercidicus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.1 | Ferredoxin | - |
Streptomyces sp. | |
1.14.14.1 | Ferredoxin | - |
Streptomyces tubercidicus |