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Literature summary extracted from
- Directed evolution of a thermostable phosphite dehydrogenase for NAD(P)H regeneration (2005), Appl. Environ. Microbiol., 71, 5728-5734.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.20.1.1 | biotechnology | evaluation of enzyme mutants for regeneration of reduced cofactors NADH and NADPH in industrial processes and comparison with Candida boidinii formate dehydrogenase | Stutzerimonas stutzeri |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.20.1.1 | I150F | thermostable mutant, half-life at 45°C 7.0 min compared to 1.4 min of wild-type | Stutzerimonas stutzeri |
| 1.20.1.1 | Q132R | thermostable mutant, half-life at 45°C 2.3 min compared to 1.4 min of wild-type | Stutzerimonas stutzeri |
| 1.20.1.1 | Q132R/Q137R/I150F/Q215L/R275Q | thermostable mutant, half-life at 45°C 161 min compared to 1.4 min of wild-type | Stutzerimonas stutzeri |
| 1.20.1.1 | Q137R | thermostable mutant, half-life at 45°C 3.8 min compared to 1.4 min of wild-type | Stutzerimonas stutzeri |
| 1.20.1.1 | Q137R/I150F/Q215L/R275Q | thermostable mutant, half-life at 45°C 200 min compared to 1.4 min of wild-type | Stutzerimonas stutzeri |
| 1.20.1.1 | Q137R/I150F/Q215L/R275Q/A319E | thermostable mutant, half-life at 45°C 567 min compared to 1.4 min of wild-type | Stutzerimonas stutzeri |
| 1.20.1.1 | Q137R/I150F/Q215L/R275Q/D162N/V315A | thermostable mutant, half-life at 45°C 614 min compared to 1.4 min of wild-type | Stutzerimonas stutzeri |
| 1.20.1.1 | Q137R/I150F/Q215L/R275Q/L276Q | thermostable mutant, half-life at 45°C 437 min compared to 1.4 min of wild-type | Stutzerimonas stutzeri |
| 1.20.1.1 | Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A | thermostable mutant, half-life at 45°C 1421 min compared to 1.4 min of wild-type | Stutzerimonas stutzeri |
| 1.20.1.1 | Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/A325V | thermostable mutant, half-life at 45°C 2315 min compared to 1.4 min of wild-type | Stutzerimonas stutzeri |
| 1.20.1.1 | Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/E130K | thermostable mutant, half-life at 45°C 1515 min compared to 1.4 min of wild-type | Stutzerimonas stutzeri |
| 1.20.1.1 | Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/I313L | thermostable mutant, half-life at 45°C 1765 min compared to 1.4 min of wild-type | Stutzerimonas stutzeri |
| 1.20.1.1 | Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/Q132R | thermostable mutant, half-life at 45°C 2350 min compared to 1.4 min of wild-type | Stutzerimonas stutzeri |
| 1.20.1.1 | Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/Q132R/V71I/E130K/I313L/A325V | thermostable mutant, half-life at 45°C 8440 min compared to 1.4 min of wild-type | Stutzerimonas stutzeri |
| 1.20.1.1 | Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/V71I | thermostable mutant, half-life at 45°C 2000 min compared to 1.4 min of wild-type | Stutzerimonas stutzeri |
| 1.20.1.1 | Q215L | thermostable mutant, half-life at 45°C 8.7 min compared to 1.4 min of wild-type | Stutzerimonas stutzeri |
| 1.20.1.1 | R275Q | thermostable mutant, half-life at 45°C 4.6 min compared to 1.4 min of wild-type | Stutzerimonas stutzeri |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.20.1.1 | 0.04 | - |
NAD+ | mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/Q132R/V71I/E130K/I313L/A325V, pH 7.3, 25°C | Stutzerimonas stutzeri |  |
| 1.20.1.1 | 0.045 | - |
phosphite | mutant Q132R, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 0.046 | - |
NAD+ | mutant Q132R/Q137R/I150F/Q215L/R275Q, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 0.046 | - |
phosphite | mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/Q132R/V71I/E130K/I313L/A325V, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 0.048 | - |
phosphite | mutant Q137R, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 0.05 | - |
NAD+ | mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/A325V, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 0.052 | - |
NAD+ | mutant Q137R/I150F/Q215L/R275Q/A319E, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 0.056 | - |
NAD+ | mutant Q137R/I150F/Q215L/R275Q/L276Q, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 0.057 | - |
NAD+ | mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/Q132R, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 0.057 | - |
phosphite | wild-type, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 0.058 | - |
phosphite | mutant Q215L, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 0.06 | - |
NAD+ | mutant Q132R, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 0.063 | - |
NAD+ | mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/V71I, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 0.064 | - |
NAD+ | mutant Q215L, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 0.065 | - |
NAD+ | mutant Q137R/I150F/Q215L/R275Q/D162N/V315A, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 0.066 | - |
NAD+ | wild-type, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 0.066 | - |
NAD+ | mutant Q137R, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 0.066 | - |
NAD+ | mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 0.07 | - |
NAD+ | mutant R275Q, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 0.071 | - |
NAD+ | mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/I313L, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 0.074 | - |
NAD+ | mutant Q137R/I150F/Q215L/R275Q, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 0.075 | - |
NAD+ | mutant I150F, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 0.075 | - |
NAD+ | mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/E130K, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 0.075 | - |
phosphite | mutant Q132R/Q137R/I150F/Q215L/R275Q, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 0.078 | - |
phosphite | mutant R275Q, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 0.092 | - |
phosphite | mutant Q137R/I150F/Q215L/R275Q/A319E, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 0.096 | - |
phosphite | mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 0.099 | - |
phosphite | mutant I150F, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 0.105 | - |
phosphite | mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/V71I, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 0.12 | - |
phosphite | mutant Q137R/I150F/Q215L/R275Q/L276Q, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 0.123 | - |
phosphite | mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/E130K, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 0.135 | - |
phosphite | mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/A325V, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 0.144 | - |
phosphite | mutant Q137R/I150F/Q215L/R275Q, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 0.155 | - |
phosphite | mutant Q137R/I150F/Q215L/R275Q/D162N/V315A, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 0.156 | - |
phosphite | mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/I313L, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 0.169 | - |
phosphite | mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/Q132R, pH 7.3, 25°C | Stutzerimonas stutzeri | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.20.1.1 | Stutzerimonas stutzeri | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.20.1.1 | phosphite + H2O + NAD+ | - |
Stutzerimonas stutzeri | phosphate + NADH + H+ | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.20.1.1 | 39 | - |
wild-type, melting temperature | Stutzerimonas stutzeri |
| 1.20.1.1 | 59.3 | - |
mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/Q132R/V71I/E130K/I313L/A325V, melting temperature | Stutzerimonas stutzeri |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.20.1.1 | 2.8 | - |
NAD+ | mutant Q132R/Q137R/I150F/Q215L/R275Q, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 2.85 | - |
NAD+ | mutant Q137R/I150F/Q215L/R275Q/D162N/V315A, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 2.9 | - |
NAD+ | mutant Q137R/I150F/Q215L/R275Q/L276Q, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 2.9 | - |
NAD+ | mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 3.13 | - |
NAD+ | mutant Q132R, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 3.2 | - |
NAD+ | mutant Q137R/I150F/Q215L/R275Q/A319E, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 3.2 | - |
NAD+ | mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/Q132R, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 3.2 | - |
NAD+ | mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/Q132R/V71I/E130K/I313L/A325V, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 3.5 | - |
NAD+ | mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/V71I, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 3.5 | - |
NAD+ | mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/E130K, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 3.5 | - |
NAD+ | mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/I313L, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 3.5 | - |
NAD+ | mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/A325V, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 3.6 | - |
NAD+ | mutant Q137R/I150F/Q215L/R275Q, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 3.96 | - |
NAD+ | mutant Q132R, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 4.1 | - |
NAD+ | mutant R275Q, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 4.4 | - |
NAD+ | wild-type, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 4.4 | - |
NAD+ | mutant I150F, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 4.6 | - |
NAD+ | mutant Q215L, pH 7.3, 25°C | Stutzerimonas stutzeri | |
| 1.20.1.1 | 4.75 | - |
NAD+ | mutant Q137R, pH 7.3, 25°C | Stutzerimonas stutzeri | |
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