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Literature summary extracted from

  • Antonyuk, L.P.
    Glutamine synthetase of the rhizobacterium Azospirillum brasilense: Specific features of catalysis and regulation (2007), Appl. Biochem. Microbiol., 43, 244-249.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.1.2 glutamate in the presence of Mg2+ the activity increases with the concentration of the substrate Azospirillum brasilense
6.3.1.2 NH4+ in the presence of Mg2+ the activity increases with the concentration of the substrate Azospirillum brasilense

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.1.2 L-alanine decreases the enzyme activity considerably Azospirillum brasilense
6.3.1.2 L-arginine inhibitory at high concentrations Azospirillum brasilense
6.3.1.2 L-glutamate in the presence of Mn2+ the activity decreases when exceeding a concentration of 10 mM glutamate Azospirillum brasilense
6.3.1.2 L-glutamine inhibitory at high concentrations Azospirillum brasilense
6.3.1.2 L-Glycine decreases the enzyme activity considerably Azospirillum brasilense
6.3.1.2 L-serine decreases the enzyme activity considerably Azospirillum brasilense
6.3.1.2 NH4+ in the presence of Mn2+ the activity decreases when exceeding a concentration of 20 mM NH4+ Azospirillum brasilense

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.1.2 0.0025
-
 L-Glu in the presence of Mg2+ Azospirillum brasilense
6.3.1.2 0.042
-
 NH4+ in the presence of Mg2+ Azospirillum brasilense
6.3.1.2 0.2
-
 ATP in the presence of Mg2+ Azospirillum brasilense

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.1.2 Co2+ supports the enzymatic activity Azospirillum brasilense
6.3.1.2 Mg2+ supports the enzymatic activity Azospirillum brasilense
6.3.1.2 Mn2+ supports the enzymatic activity Azospirillum brasilense

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.2 Azospirillum brasilense
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.2 ATP + L-Glu + NH4+
-
 Azospirillum brasilense ADP + phosphate + L-Gln
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.1.2 dodecamer type alpha12 dodecamer with oligomer and monomer having molecular weights of 630000 and 52000 Da, respectively Azospirillum brasilense

Synonyms

EC Number Synonyms Comment Organism
6.3.1.2 GSI
-
 Azospirillum brasilense

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6.3.1.2 6.25
-
 in the presence of Mn2+, the enzyme is active within a relatively narrow range of weakly acidic pH (no higher than 6.25) and inactive at alkaline pH Azospirillum brasilense
6.3.1.2 6.5 9.75 with Mg2+ GS is active within a broad range of physiological pH values (6.5-9.75) Azospirillum brasilense

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.1.2 6.25
-
 in the presence of Mn2+, at optimum pH values, moderately adenylated GS is twice more active in the presence of Mn2+ (at pH 6.25) than Mg2+ (at pH 8.0), whereas, at pH 7.1, which corresponds to the intracellular level of pH for the enzyme is almost equiactive with either cation Azospirillum brasilense
6.3.1.2 8
-
 in the presence of Mg2+, at optimum pH values, moderately adenylated GS is twice more active in the presence of Mn2+ (at pH 6.25) than Mg2+ (at pH 8.0), whereas, at pH 7.1, which corresponds to the intracellular level of pH for the enzyme is almost equiactive with either cation Azospirillum brasilense