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Literature summary extracted from

  • Wang, Y.J.; Zheng, Y.G.; Zheng, R.C.; Shen, Y.C.
    Stability study on the nitrile hydratase of Nocardia sp. 108: From resting cells to crude enzyme preparation (2006), Appl. Biochem. Microbiol., 42, 384-387.
No PubMed abstract available

General Stability

EC Number General Stability Organism
4.2.1.84 KH2PO4-NaOH and Tris-HCl buffer are tested at 28°C and pH 7.5. 20% NHase activity is lost after 18 h and is further decreasing. 73.5 h later, NHase activity in Tris-HCl is unchanged while in KH2PO4-NaOH abrupt decrease is observed. Nocardia sp.

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.84 Nocardia sp.
-
-
-
4.2.1.84 Nocardia sp. 108
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.84 (NH4)2SO4 fractionation Nocardia sp.

Storage Stability

EC Number Storage Stability Organism
4.2.1.84 4°C, Tris-HCl buffer pH 7.2 Nocardia sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.84 acrylonitrile + H2O
-
 Nocardia sp. acrylamide
-
 ?
4.2.1.84 acrylonitrile + H2O
-
 Nocardia sp. 108 acrylamide
-
 ?

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.1.84 4 24
-
 Nocardia sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.84 7 7.5
-
 Nocardia sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.84 6 8
-
 Nocardia sp.