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Literature summary extracted from

  • Rani, D.N.; Abraham, T.E.
    Kinetics and thermal stability of two peroxidase isozymes from Eupatorium odoratum (2006), Appl. Biochem. Biotechnol., 128, 215-226.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.11.1.7 lyophilized peroxidase remains stable and active without any loss of activity for more than 2 years Chromolaena odorata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.7 0.016
-
 H2O2 30°C, pH 5.5, cosubstrate: pyrogallol, isoenzyme E5 Chromolaena odorata
1.11.1.7 0.21
-
 reduced 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid) 30°C, pH 5.5, isoenzyme E5 Chromolaena odorata
1.11.1.7 0.28
-
 H2O2 30°C, pH 5.5, cosubstrate: o-phenylenediamine, isoenzyme E5 Chromolaena odorata
1.11.1.7 0.35
-
 H2O2 30°C, pH 5.5, cosubstrate: reduced 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid), isoenzyme E5 Chromolaena odorata
1.11.1.7 9
-
 o-phenylenediamine 30°C, pH 5.5, isoenzyme E5 Chromolaena odorata
1.11.1.7 55.4
-
 pyrogallol 30°C, pH 5.5, isoenzyme E5 Chromolaena odorata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.7 43000
-
 x * 43000, isoenzyme E5, SDS-PAGE Chromolaena odorata

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.7 Chromolaena odorata
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.11.1.7 glycoprotein isoenzyme E5 contains 25% w/w carbohydrate Chromolaena odorata

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.7
-
 Chromolaena odorata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.1.7 leaf
-
 Chromolaena odorata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.7 8987
-
 isoenzyme E5 Chromolaena odorata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.7 additional information negligible activity with guaiacol, isoenzyme E5 Chromolaena odorata ?
-
 ?
1.11.1.7 o-phenylenediamine + H2O2 isoenzyme E5 Chromolaena odorata ?
-
 ?
1.11.1.7 pyrogallol + H2O2 isoenzyme E5 Chromolaena odorata purpurogallin + H2O
-
 ?
1.11.1.7 reduced 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid) + H2O2 isoenzyme E5 Chromolaena odorata oxidized 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid) + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.7 ? x * 43000, isoenzyme E5, SDS-PAGE Chromolaena odorata

Synonyms

EC Number Synonyms Comment Organism
1.11.1.7 peroxidase isoenzyme E5
-
 Chromolaena odorata

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.7 40
-
 half-life: 239.1 h, isoenzyme E5 Chromolaena odorata
1.11.1.7 50
-
 half-life: 52.2 h, isoenzyme E5 Chromolaena odorata
1.11.1.7 60
-
 half-life: 15.8 h, isoenzyme E5 Chromolaena odorata
1.11.1.7 65
-
 half-life: 2.8 h, isoenzyme E5 Chromolaena odorata

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.7 102.5
-
 pyrogallol 30°C, pH 5.5, isoenzyme E5 Chromolaena odorata
1.11.1.7 744.6
-
 o-phenylenediamine 30°C, pH 5.5, isoenzyme E5 Chromolaena odorata
1.11.1.7 2383
-
 reduced 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid) 30°C, pH 5.5, isoenzyme E5 Chromolaena odorata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.7 3.6 3.8 isoenzyme E5 Chromolaena odorata

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.11.1.7 Chromolaena odorata isoenzyme E5, isoelectric focusing
-
 5