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Literature summary extracted from

  • Argueello-Morales, M.; Sanchez-Gonzalez, M.; Canedo, M.; Quirasco, M.; Farres, A.; Lopez-Munguia, A.
    Proteolytic modification of Leuconostoc mesenteroides B-512F dextransucrase (2005), Antonie van Leeuwenhoek, 87, 131-141.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.5 sucrose the 155000 Da enzyme form is more sensitive to substrate inhibition than the 170000 Da precursor form Leuconostoc mesenteroides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.5 29
-
 sucrose 30°C, pH 5.2, 170000 Da precursor Leuconostoc mesenteroides
2.4.1.5 34
-
 sucrose 30°C, pH 5.2, 155000 Da enzyme form Leuconostoc mesenteroides

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.5 Leuconostoc mesenteroides
-
-
-
2.4.1.5 Leuconostoc mesenteroides B-512F
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.5 proteolytic modification change of molecular mass of the enzymeincubated for 60 days at room temperature. After storage, the 170000 Da dextransucrase precursor decreases in concentration as a consequence of proteolysis. Initially a mixture of the precursor and a 155000 Da form are found. After 60 days of incubation, the 155000 form is further digested to lower molecular mass species, particularly a 129000 Da form, which still retains dextransucrase activity Leuconostoc mesenteroides

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.5 141
-
 155000 Da enzyme form Leuconostoc mesenteroides
2.4.1.5 145
-
 170000 Da precursor Leuconostoc mesenteroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.5 sucrose + (1,6-alpha-D-glucosyl)n
-
 Leuconostoc mesenteroides D-fructose + (1,6-alpha-D-glucosyl)n+1
-
 ?
2.4.1.5 sucrose + (1,6-alpha-D-glucosyl)n
-
 Leuconostoc mesenteroides B-512F D-fructose + (1,6-alpha-D-glucosyl)n+1
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.5 additional information
-
 additional information
-
 Leuconostoc mesenteroides