Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kedar, G.C.; Brown-Driver, V.; Reyes, D.R.; Hilgers, M.T.; Stidham, M.A.; Shaw, K.J.; Finn, J.; Haselbeck, R.J.
    Evaluation of the metS and murB loci for antibiotic discovery using targeted antisense RNA expression analysis in Bacillus anthracis (2007), Antimicrob. Agents Chemother., 51, 1708-1718.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
6.1.1.10 pharmacology the enzyme is an important antibiotic target Bacillus anthracis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.10 genes metS1 and metS2, library construction, subcloning in Escherichia coli strains DH5alpha and INV110 Bacillus anthracis

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.10 additional information gene replacement mutagenesis, downregulation of metS1 by its antisense construct, xylose-induced antisense expression, antisense orientation is identified for the metS1 allele, while no such orientation bias is seen for the metS2 allele, attenuation of MetS1 enzyme expression hypersensitizes Bacillus anthracis cells to a MetS-specific antimicrobial compound Rx-000019, but not to other antibiotics that affect cell wall assembly, fatty acid biosynthesis, protein translation, or DNA replication, overview Bacillus anthracis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.10 Mg2+
-
 Bacillus anthracis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.10 ATP + L-methionine + tRNAMet Bacillus anthracis essentiality of the metS1, nonessentiality of the metS2 for vegetative growth AMP + diphosphate + L-methionyl-tRNAMet
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.10 Bacillus anthracis
-
 UM23C1-1and GRI strains, genes metS1 and metS2
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.10 ATP + L-methionine + tRNAMet
-
 Bacillus anthracis AMP + diphosphate + L-methionyl-tRNAMet
-
 ?
6.1.1.10 ATP + L-methionine + tRNAMet essentiality of the metS1, nonessentiality of the metS2 for vegetative growth Bacillus anthracis AMP + diphosphate + L-methionyl-tRNAMet
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.10 methionyl-tRNA synthetase
-
 Bacillus anthracis
6.1.1.10 MetS
-
 Bacillus anthracis

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.10 ATP
-
 Bacillus anthracis